实验动物与比较医学2017，Vol.37Issue(6)：434-441,8.DOI:10.3969/j.issn.1674-5817.2017.06.003
应用微卫星DNA标记对福建亚种猕猴遗传多样性的分析
Analysis on Genetic Diversity of Macaca mulatta littoralis by Using Microsatellite DNA Markers
摘要
Abstract
Objective To analyze the genetic diversity ofMacaca mulatta littoralis and providing basic data for genetic quality monitoring methods of the population.Methods Twenty-eight Macaca mulatta littoralis were genotyped by using 20 microsatellite DNA markers,DNA was extracted from blood and amplified by PCR,and PCR products were analyzed by non-modified polyacrylamide gel electrophoresis.Every microsatellite locus was calculated by software POPGENE 1.32 and Excel.Results All 20 microsatellite loci were highly polymorphic,200 alleles were found in the population.The number of alleles (Na),effective number of alleles (Ne),expected heterozygosity (He),observed heterozygosity (Ho),Sharnon's information index (Ⅰ) and polymorphism information content (PIC) were ranged from 5 to 16,3.7333 to 11.6148,0.7455 to 0.9305,0.3214 to 0.7500,1.3998 to 2.5963,and 0.6862 to 0.9074,with an average of 10,6.8438,0.8509,0.4607,2.0166 and 0.8154,respectively.All of 17 loci showed significant deviations from Hardy-Weinberg equilibrium.Conclusions There are high genetic diversity in Macaca mulatta littoralis of this colony.The experiments can provide a theoretical foundation for establishing genetic quality monitoring method with specific microsatellite loci.关键词
微卫星标记/福建亚种猕猴/遗传多样性Key words
Microsatellite marker/Macaca mulatta littoralis/Genetic diversity分类
生物科学引用本文复制引用
周建华,李志雄,杨燕燕,谢金东,俞春英,王训立..应用微卫星DNA标记对福建亚种猕猴遗传多样性的分析[J].实验动物与比较医学,2017,37(6):434-441,8.基金项目
福建省实验动物研究重点项目(2014Y0080) （2014Y0080）
