实验动物与比较医学2017，Vol.37Issue(6)：442-447,6.DOI:10.3969/j.issn.1674-5817.2017.06.004
近交系长爪沙鼠生化标记遗传监测方法的建立及其应用
Establishment and Application of Genetic Monitoring Methods for Inbred Gerbil by Biochemical Markers
摘要
Abstract
Objective To establish genetic monitoring method by biochemical markers for inbred gerbils and apply it in analyzing the homogenous of the ischemia-prone inbred gerbil lines CMU/1 and CMU/2.Methods Twenty-six biochemical marker loci were selected to perform cellulose acetate fiber electrophoresis for several kinds of gerbil tissues by optimum electrophoresis conditions referred to previous report and optimized the sample treatment and staining method.Then these methods were used in detecting homogeneous of 2 inbred lines CMU/1 (77 gerbils) and CMU/2 (44 gerbils) genetic quality involved generation F21-F23.Results All of 26 biochemical marker loci could be detected successfully in both inbred gerbil of 121 gerbils.Thereinto,24 loci exhibited monomorphism within and between CMU/1 and CMU/2.However,the loci Es-3 and Es-4 showed polymorphism between two strains.Conclusion The biochemical marker method for genetic monitoring of inbred gerbil has been successfully established.The 26 biochemical marker loci for inbred gerbil strain CMU/1 and CMU/2 has been confirmed which homogeneous reached to 100％.These data indicated that two inbred strains match the standard of inbred laboratory animals (GB14923-2010).关键词
长爪沙鼠/近交系/脑缺血模型/生化位点标记/遗传监测Key words
Gerbil/Inbred strain/Ischemia model/Biochemical marker locus/Genetic monitoring分类
生物科学引用本文复制引用
李迎,杜小燕,陈振文,马兰芝,尚世臣,尚玉璞,赵权,李桂军,崔晓霞,王冬平..近交系长爪沙鼠生化标记遗传监测方法的建立及其应用[J].实验动物与比较医学,2017,37(6):442-447,6.基金项目
国家科技支撑计划课题(2015BAI09B01-04) （2015BAI09B01-04）
