实验动物与比较医学2017，Vol.37Issue(6)：448-454,7.DOI:10.3969/j.issn.1674-5817.2017.06.005
兔斯氏艾美耳球虫荧光定量PCR检测方法的建立
Detection of Eimeria Stiedai by Real-Time PCR
摘要
Abstract
Objective To develop the method for quantitatively detecting Eimeria stiedai (E.stiedai) by Real-Time PCR (RT-PCR).Methods Specific primers were designed and synthesized according to a part of the sequence of ITS1 region ofE.stiedai.The recombinant plasmid was constructed to established the standard curve of RT-PCR.The specificity,sensitivity and reproducibility of the RT-PCR method were performed.Result The method was specific and sensitive for detection ofE.stiedai.Meanwhile,the sensitivity of the present method could detect the DNA of one E.stiedai and the variation coefficients of intra-assay and inter-assay were less than 2.0％ respectively.Conclusion The established RT-PCR is a specific,sensitive and reliable method for the quantitative detection ofE.stiedai.关键词
兔斯氏艾美耳球虫/荧光定量PCR/检测Key words
E.stiedai/Real-Time PCR(RT-PCR)/Detection分类
农业科技引用本文复制引用
温福利,郑和平,党源,薛来恩,张诗兰..兔斯氏艾美耳球虫荧光定量PCR检测方法的建立[J].实验动物与比较医学,2017,37(6):448-454,7.基金项目
福建省科技计划引导性项目(2015Y0075)和南京军区福州总医院临床应用研究专项(2015L01) （2015Y0075）
