实用医学杂志2018，Vol.34Issue(9)：1441-1444,4.DOI:10.3969/j.issn.1006-5725.2018.09.009
含七氟醚HTK液对心脏移植离体鼠心电生理的保护机制
Protective Mechanism of containing sevoflurane HTK Solution on Cardiac Electrophysiology in Isolated Rat Cardiac Transplantation
摘要
Abstract
Objective To investigate the electrophysiological protective effect of HTK solution containing sevoflurane on rat cardiac transplantation. Methods Twenty-four male Sprague-Dawley rats were divided into the control group,sevoflurane group and Heptanol group. Rat hearts in 3 groups were stored in HTK solution,containing sevoflurane and sevoflurane+heptanol HTK solution for 6 h. Heart resuscitation time,the duration of arrhythmia and monophasic action potential(MAP)and heart rate(HR)at different time points were recorded.Monophasic action potential duration of repolarization at 50% and 90%(MAPD50 and MAPD90),monophasic action potential amplitude(MAPA)and maximal velocity(Vmax)were analyzed. Results The isolated rat hearts in each group can be successfully restored to the spontaneous heart beat. Compared with group C ,heart resuscitation time in group S and group H was significantly shortened,heart rate(HR)was significantly decreased at T1,MAPD50 and MAPD90 in heart intima and epicardium were shortened at T1 ~ T2,the incidence of ventricular fibrillation and reperfusion arrhythmia Scores were reduced,with the shorter duration of ventricular fibrillation(P<0.05). No significant difference was found in Vmax and MAPA among the three groups at each time point. Conclusion HTK solution containing sevoflurane and HTK solution containing heptanol had similar electrophysiological effects ,which could inhibit the prolonged monophasic action potential(MAP)and reduce arrhythmia. The electrophysiological protective effect of HTK solution containing sevoflurane may be associated with the inhibition of gap junction function.关键词
七氟醚/HTK液/心脏移植/单相动作电位/缝隙连接/再灌注心律失常Key words
sevoflurane/htk solution/heart transplantation/monophasic action potential during/gap junction/reperfusion arrhythmia引用本文复制引用
李伟超,高巨,高鸿,佘玉琦,王子君,刘艳秋..含七氟醚HTK液对心脏移植离体鼠心电生理的保护机制[J].实用医学杂志,2018,34(9):1441-1444,4.基金项目
2017年度贵阳市人民政府-贵州医科大学科学技术联合基金资助项目(编号:201730-42) （编号:201730-42）
麻醉学专业学位研究生工作站基金资助项目(编号:080800818) （编号:080800818）
