实用医学杂志2018，Vol.34Issue(10)：1589-1593,1597,6.DOI:10.3969/j.issn.1006-5725.2018.10.003
维吾尔族不同HER-2表型乳腺癌中Survivin和Caspase-9基因表达的相关性及其意义
Correlation and clinical significance of Survivin and Caspase-9 gene expression in Uygur breast cancer patients with different HER-2 phenotypes
摘要
Abstract
Objective To detect the expression of Survivin and Caspase-9 gene in Uygur breast cancer pa-tients with different HER-2 phenotypes,to find out the difference and association of the two genes and to find out the potential roles of the two genes breast cancer pathogenesis. Methods We selected 72 Uygur patients diag-nosed as breast cancer initially and they were divided into group A with HER-2 positive(n = 39)and group B with HER-2 negative(n = 33). Another 40 Uygur patients with benign breast were involved as the controls. Immunohis-tochemistry and real-time RT-PCR were used to detect the two genes,and analyze the differences and association of each gene between the groups. Results (1)The expression of Survivin gene in group A and B were higher than that in the control group. Further analysis showed that the expression of Survivin gene was enhanced in group A when compared with that in group B(P < 0.05);while even the expression of Survivin gene in group B was higher than that in the control group but no statistical difference was found(P > 0.05).(2)The expression of Caspase-9 gene in group A and B were lower than that in the control group. Real-time RT-PCR showed that the expression of Caspase-9 gene of group A was decreased when compared with that in group B(P < 0.05);While the expression of Caspase-9 gene of group B was slightly lower that of the control group but it showed no statistical significance (P > 0.05). Immunohistochemical results showed there were no statistical differences of expression of Caspase- 9 gene in group A and B and control group(all P > 0.05). The expression of Survivin and Caspase-9 gene was nega-tively associated in group A and B(P < 0.01;P < 0.05). Conclusions In Uygur patients with HER-2 positive breast cancer,the expression of Survivin gene is enhanced but that of caspase-9 gene is decreased,and they are negatively associated. Through inhabiting caspase-9 gene,Survivin gene may potentially lead to the occurrence of HER-2 positive breast cancer.关键词
乳腺癌/Survivin/Caspase-9/HER-2/维吾尔族Key words
breast cancer/Survivin/Caspase-9/HER-2/Uygur引用本文复制引用
石文龙,巫姜,李炳军,李南林,凌瑞,陈江浩..维吾尔族不同HER-2表型乳腺癌中Survivin和Caspase-9基因表达的相关性及其意义[J].实用医学杂志,2018,34(10):1589-1593,1597,6.基金项目
国家自然科学基金项目(编号:81472598) （编号:81472598）
