物理化学学报2018，Vol.34Issue(2)：177-184,8.DOI:10.3866/PKU.WHXB201707121
基于氨基与表面乙烯砜基反应动力学调控配基表面密度
Control of the Ligand Surface Density through Reaction Kinetics of Amino and Surface Vinyl Sulfone Groups
摘要
Abstract
Control over the ligand surface density provides an accurate molecular basis for the quantitative study of biomolecular interactions. However, the classic hybrid self-assembly method lacks general applicability toward different self-assembly systems. In this paper, we report a new method based on the reaction kinetics of vinyl sulfone groups presented on surface to control the surface ligand density.Nα,Nα-bis(carboxymethyl)-L-lysine(ab-NTA)was selected as the model biological ligand and the catalyst for surface reaction was screened. The surface reaction was characterized by X-ray photoelectron spectroscopy (XPS) and the surface membrane potential. Static water contact angle was used to quantify the kinetics of the surface reaction, and calculations showed that the rate constant was 0.0012 min?1. The ability of the biological functional surface to bind a histidine labeling protein (SA-6His) was investigated by surface plasmon resonance (SPR). The results show that such a surface has a higher protein binding quantity and binding strength than the traditional NHS-NTA surface. Four biological functional surfaces with different ligand densities were prepared by controlling the reaction time and catalyst, and the protein static adsorption of these surfaces was analyzed by SPR. The results show that ligand density and multivalence of the biological functional surface can be controlled by modulating the reaction time and catalyst.关键词
表面催化/乙烯基砜/ab-NTA/密度控制/SPR/多价态Key words
Surface catalysis/Vinyl sulfone/ab-NTA/Density control/SPR/Multivalent分类
化学化工引用本文复制引用
程昉,李明洋,何炜,王汉奇..基于氨基与表面乙烯砜基反应动力学调控配基表面密度[J].物理化学学报,2018,34(2):177-184,8.基金项目
中央高校基本科研业务费(DUT16RC(3)019)和青年千人计划资助项目 （DUT16RC(3）
Fundamental Research Funds for the Central Universities,China(DUT16RC(3)019)and Recruitment Program of Global Youth Experts,China. （DUT16RC(3）
