陕西科技大学学报2018，Vol.36Issue(3)：46-51,62,7.
牛乳酪蛋白双联胶体金免疫层析试纸条的研制
Preparation of double-layered colloidal gold immunochromatographic test strip of milk casein
摘要
Abstract
Aduteration of raw bovine milk to goat milk causes damage to food safety of its products ,especially to consumers with bovine milk allergy .The purpose of this study was to establish a method of immunochromatographic strips to detect adulteration of raw goat milk . Bovine milk αs1-CN (αs1-Casein) and milkβ-LG (β-Lactoglobulin) was taking as the test ob-ject to develop double the colloidal gold immunochromatographic strip .Their specific ploy-clonal antibodies was prepared .The protein concentrations of polyclonal antibodies of α-CN IgG andβ-LG IgG were 4 .86 mg/mL and 5 .99 mg/mL and the titers of these two antibodies were 1:25600 and 1 : 204800 respectively .In the preparation experiments of colloidal gold ,the optimal pH was 8 .0 ,and the best protein labeling amount was 10 μL ,and the opti-mal incorporation ratio of α-CN IgG inβ-LG IgG was 20% .Test strips were coated with glass fiber VL68 as gold standard and Sarteries CN 95 as nitrocellulose NC membranes .NC mem-brane was coated with 6 μL ,1 .0 mg/mL α-CN IgG and 6 μL ,1 .0 mg/mL β-LG IgG on Tlines ,and use 6 μL ,1 .0 mg/mL secondary antibody to coated C line as well .The test strip can detect α-CN andβ-LG with no obvious cross-reaction ,and the lowest detectable content of milk is 5% .关键词
牛羊乳掺假/快速检测/胶体金免疫层析Key words
adulteration of goat milk with bovine milk/rapid detection/colloidal gold immu-nochromatography分类
轻工纺织引用本文复制引用
薛海燕,许淼,白文卿,张颖,薛丽欢..牛乳酪蛋白双联胶体金免疫层析试纸条的研制[J].陕西科技大学学报,2018,36(3):46-51,62,7.基金项目
国家自然科学基金项目(31301405) （31301405）
陕西省科技厅科技统筹创新工程计划项目(2013KTZB02-02-05(2)) （2013KTZB02-02-05(2）
陕西省大学生创新创业训练计划项目(1344) （1344）
