中国草食动物科学2018，Vol.38Issue(3)：1-6,6.DOI:10.3969/j.issn.2095-3887.2018.03.001
牦牛SCD基因的克隆和生物信息学分析
Cloning and Bioinformatics Analysis of SCD Gene in Yak
摘要
Abstract
In this paper,the biological function of stearyl coenzyme A dehydrogenase(SCD)gene of new polled breed yak was studied, CDS area of SCD gene primer was designed and gene was cloned,the analysis tool in bioinformatics was EMBOSS and bioinformatics analysis program. The results showed that the length of the CDS area of the SCD gene was 1 080 bp and coded 359 amino acid residues in the protein. Compared to SCD gene sequence from common cattle,there were7 base changes in yak SCD gene,including 1 substitutions for A-C,2 substitutions for C-A,1 transitions for A-G,2 transitions for C-T,1 substitutions for T-A,respectively. In addition,amino acid sequences only changed at 3 sites in the first,sixth and seventh sites.The formulas for yak SCD gene encoding protein was C1922H2912N506O514S11,molecular weight was about 41.7 kD,theoretical oelectric point(pI)was 9.23,extinction coefficient was 83895,not stability coefficient was 44.41,the hydrophobic index was 86.96,average hydrophilicity was -0.235,belongsed to the unstable soluble alkaline protein,in mammals,reticulocyte had a half-life of 30 h. The secondary structure consists of alpha helices,beta folding,extension chains and random curl structures. The protein also had low complex structure and transmembrane structure.关键词
牦牛/不饱和脂肪酸合成酶/基因克隆/生物信息学分析Key words
yak/SCD/gene cloning/bioinformatics analysis分类
农业科技引用本文复制引用
付东海,梁春年,吴晓云,王宏博,褚敏,郭宪,裴杰,包鹏甲,丁学智,阎萍..牦牛SCD基因的克隆和生物信息学分析[J].中国草食动物科学,2018,38(3):1-6,6.基金项目
中央级公益性科研院所基本科研业务费专项资金(1610322017002) （1610322017002）
甘肃省草食畜产业技术体系 （）
现代肉牛牦牛产业技术体系(CARS-37) （CARS-37）
中国农业科学院牦牛资源与育种创新团队(CAAS-ASTIP-2014-LIHPS) （CAAS-ASTIP-2014-LIHPS）
