中国草食动物科学2018，Vol.38Issue(3)：7-11,5.DOI:10.3969/j.issn.2095-3887.2018.03.002
SCNT牛胚胎中PWS/AS基因簇母源印记控制区甲基化模式研究
Methylation Pattern of PWS/AS Imprinting Cluster in Clone Embryos
摘要
Abstract
In order to study the methylation pattern of clone embryos,early clone embryos at five developmental stages (gamophase, S phase,2-cell,8-cell and morula)were selected to study the methylation pattern of PWS/AS imprinting cluster by bisulfite sequencing PCR (BSP). The results of sequencing analysis indicated that demethylation pattern of genome was affected by the early methylation reconstruction of SCNT embryos. There were two CPG positions that were respectively the first and the fifth CPG position located at 258 bp and 1693 bp region on upstream of SNRPN transcriptional start.关键词
甲基化/印记基因/SNRPN基因/启动子Key words
methylation/imprinted gene/SNRPN gene/promoter分类
农业科技引用本文复制引用
王巍,方东辉,付茂忠,甘佳,唐慧,石溢,王淮,易军..SCNT牛胚胎中PWS/AS基因簇母源印记控制区甲基化模式研究[J].中国草食动物科学,2018,38(3):7-11,5.基金项目
"十二五"农村领域国家科技计划课题(2015BAD03B04-3) （2015BAD03B04-3）
四川省科技支撑项目(2015NZ0020) （2015NZ0020）
四川省应用基础项目(2015JY0181) （2015JY0181）
四川省育种攻关项目(2016NYZ0050) （2016NYZ0050）
