局解手术学杂志2018，Vol.27Issue(5)：318-321,4.DOI:10.11659/jjssx.11E017092
铅暴露对成骨细胞MC3T3-E1细胞线粒体的影响
Effects of lead exposure on mitochondria of osteoblast MC3T3-E1
摘要
Abstract
Objective To evaluate the effect of lead exposure on mitochondria of osteoblasts MC3T3-E1. Methods Osteoblasts MC3T3-E1 with different concentrations(0,1,10,100μmol/L)of lead acetate[Pb(Ac) 2] solution were incubated for 24h. After that,the degree of depolarization of mitochondrial membrane potential,the amount of ATP synthesis,cytochrome c content in cytoplasm and mitochon-dria and Caspase-3 activity were measured. In addition,the morphological changes of mitochondria were observed by transmission electron mi-croscope. Results Lead exposure can cause the decrease of mitochondrial membrane potential,the decrease of ATP synthesis in osteoblasts,Ithe release of cytochrome c from mitochondria to cytoplasm and the increase of Caspase-3 activity in MC3T3-E1 cells. All results are significantly dose-dependent. n addition,through electron microscopy observation,it was found that the mitochondria in exposure of 100 μmol/L lead acetate swelled obviously. Conclusion Lead exposure can cause the functional and structural damage of MC3T3-E1 mitochondria and thus result in apoptosis.关键词
铅暴露/成骨细胞/线粒体/凋亡Key words
lead exposure/osteoblast/mitochondria/apoptosis分类
医药卫生引用本文复制引用
辜洁,高瑞,周薇,王萍..铅暴露对成骨细胞MC3T3-E1细胞线粒体的影响[J].局解手术学杂志,2018,27(5):318-321,4.基金项目
国家重点实验室自主研究课题(SKLZZ201503) （SKLZZ201503）
