局解手术学杂志2018，Vol.27Issue(5)：322-325,4.DOI:10.11659/jjssx.12E017078
miR-181b通过下调CYLD蛋白影响甲状腺乳头状癌细胞的增殖和凋亡
Effects of miR-181 b on proliferation and apoptosis of thyroid papillary carcinoma cells by down-regulating CYLD protein
摘要
Abstract
Objective To investigate the effect of miR-181b on the proliferation and apoptosis of thyroid papillary carcinoma cells by down-regulating CYLD protein and its mechanism. Methods qPCR was used to detect the expression and difference of miR-181b in papilla-ry thyroid carcinoma and normal thyroid tissue. Western blotting was used to detect the regulation between miR-181b and CYLD protein. Clone formation assay was used to detect the proliferation of thyroid cancer cells after inhibition of miR-181b. Flow cytometry was used to detect the apoptosis of thyroid carcinoma cells after inhibition of miR-181b. Results Compared with normal thyroid tissue,the expression of miR-181b in papillary thyroid carcinoma was up-regulated,the difference was statistically significant(P<0. 05). The expression level of miR-181b in FTC-133 cell line was relatively high. Western blotting confirmed that miR-181b could directly regulate the expression of CYLD protein. Inhi-bition of miR-181b expression can inhibit the proliferation of thyroid cancer cells,and to some extent promote its apoptosis behavior. Conclu-sion miR-181b can regulate the expression of CLYD and affect the proliferation and apoptosis of thyroid cancer cells.关键词
miR-181b/甲状腺乳头状癌/CYLD/细胞凋亡Key words
miR-181b/thyroid papillary carcinoma/CYLD/apoptosis分类
医药卫生引用本文复制引用
王雷,于东升,赵临潇,柴丽霞..miR-181b通过下调CYLD蛋白影响甲状腺乳头状癌细胞的增殖和凋亡[J].局解手术学杂志,2018,27(5):322-325,4.基金项目
陕西省科学技术研究发展计划基金(2012SF2-02-05) （2012SF2-02-05）
