中国比较医学杂志2018，Vol.28Issue(5)：1-5,11,6.DOI:10.3969/j.issn.1671-7856.2018.05.001
多参数高场强磁共振成像评估恒河猴急性脑梗死血栓-溶栓模型
High-field magnetic resonance imaging with multiple parameters to evaluate the thrombus-thrombolysis model in rhesus monkeys with acute cerebral infarction
摘要
Abstract
Objective To examine the nonhuman primate(NHP)model of acute cerebral infarction thrombus-thrombolysis using multi-parameter high-field magnetic resonance imaging(MRI). Methods Altogether, 8 adult male rhesus monkeys aged 8.2(± 1.2)years old and weighing 9.4(± 1.0)kg were randomized into an infarction group(n=4)and thrombolysis group(n =4). Middle cerebral artery occlusion(MCAO)was induced with a clot in the M1 segment. Monkeys in the thrombolysis group were treated with the recombinant tissue plasminogen activator, rt-PA, while those in the infarction group were treated with 0.9% NaCl only. T2 weighted imaging(T2WI), T2-weighted-fluid-attenuated inversion recovery(T2-FLAIR), time-of-flight magnetic resonance angiography(TOF-MRA), and diffusion-weighted imaging(DWI)were used to examine all monkeys at 4 and 24 h after onset of ischemia. Results The rhesus monkey thrombus-thrombolysis model was successfully established. MRA showed that the middle cerebral artery(MCA) was not recanalized in the infarction group, but was recanalized in the thrombolysis group. T2WI sequence showed an increase in infarction volume(12 027 ± 5507 mm3)in the infarction group compared with the thrombolysis group(4910 ± 2764 mm3). DWI sequence showed an increase in infarction volume(9498 ± 5226 mm3)in the infarction group and thrombolysis group(4854 ± 1792 mm3). Both T2WI and DWI sequences showed no significant difference in infarction volume at 4 h between the two groups, while infarction volume in the thrombolysis group at 24 h was significantly lower compared with the infarction group. The increase in infarction volume was significantly lower in the thrombolysis group compared with the infarction group. Conclusions MRI sequences can be used to successfully evaluate recanalization and infarct changes in the thrombus-thrombolysis model in rhesus monkeys.关键词
恒河猴/急性脑梗死/溶栓/磁共振成像Key words
rhesus monkeys/acute cerebral infarction/thrombolysis/magnetic resonance imaging/MRI分类
医药卫生引用本文复制引用
张默,吴迪,何小夺,齐志刚,李坤成,陈健..多参数高场强磁共振成像评估恒河猴急性脑梗死血栓-溶栓模型[J].中国比较医学杂志,2018,28(5):1-5,11,6.基金项目
北京市医院管理局青苗项目(编号:QML20170802) （编号:QML20170802）
国家重点研发计划(编号:2016YFC0107107) （编号:2016YFC0107107）
北京市科学技术委员会资助(编号:Z171100000117001). （编号:Z171100000117001）
