中国比较医学杂志2018，Vol.28Issue(5)：6-11,6.DOI:10.3969/j.issn.1671-7856.2018.05.002
杏仁核快速电点燃癫痫大鼠神经元限制性沉默因子表达变化
Changes in expression of repressor element silencing transcription factor in rapid amygdala kindling epilepsy rats
摘要
Abstract
Objective To detect changes in expression of repressor element silencing transcription factor(REST) in rapid amygdala kindling epilepsy rats. Methods Adult Sprague-Dawley rats were randomly divided into a sham group (group S),epilepsy 2 h group(group EP-2 h),epilepsy 14 d group(group EP-14 d),and epilepsy 35 d group(group EP-35 d). An experimental epilepsy kindling model of the amygdala was established by electrical stimulation. After 2 h, 14 d,and 35 d,rats were perfused and their brains were fixed and frozen for coronal sections. Immunohistochemistry was used to detect expression of REST and the neuron specific nuclear protein, NeuN. Results Expression of REST was significantly increased in the CA1, CA3, and dentate gyrus regions in groups EP compared with group S(P < 0.05), particularly in group EP-2 h. In contrast,NeuN staining was significantly decreased in groups EP compared with group S. Visible neuronal loss was observed in the hippocampus of the EP-35 d group. Conclusions Up-regulation of REST in rat hippocampus may be involved in epileptogenesis.关键词
杏仁核/快速电点燃/癫痫/神经元限制性沉默因子Key words
amygdala/rapid kindling/epilepsy/repressor element silencing transcription factor/REST分类
医药卫生引用本文复制引用
呼奶英,于佳田,别会杰,王德广..杏仁核快速电点燃癫痫大鼠神经元限制性沉默因子表达变化[J].中国比较医学杂志,2018,28(5):6-11,6.基金项目
江苏省教育厅科研项目(编号:14KJB310021). （编号:14KJB310021）
