中国比较医学杂志2018，Vol.28Issue(5)：39-45,7.DOI:10.3969/j.issn.1671-7856.2018.05.008
β-tubulin 在肾脏D5R 与CCKBR 相互调节中的重要作用
Mechanistic study of β-tubulin and its interaction with the dopamine D5 receptor, cholecystokinin B receptor, and water-sodium metabolism
摘要
Abstract
Objective To examine the role of β-tubulin on the interaction between the cholecystokinin B receptor (CCKBR),dopamine D5 receptor(D5R), and water-sodium metabolism. Methods Normotensive and hypertensive renal proximal tubular cells(RPTC)were equally randomized into three separate groups: a gastrin group, fenoldopam group,and gastrin+nocodazole group. Immunofluorescence was used to determine localization of β-tubulin,CCKBR,and D5R. Western blotting was used to detect CCKBR, D5R, and Na-K-ATP expression. Results Gastrin stimulation in normotensive RPTC increased D5R expression(P < 0.05)and decreased Na-K-ATP expression(P < 0.05). These changes were blocked by a tubulin inhibitor(P < 0.05). However, interaction between CCKBR, D5R, and Na-K-ATP expression was not significantly affected in hypertensive RPTC. Immunofluorescence showed that CCKBR and D5R can induce one another,followed by transport to the plasma membrane, which can prevented by a tubulin inhibitor. Further, tubulin is disordered in hypertensive RPTC,which cannot support intracellular CCKBR and D5R transport. Conclusions tubulin plays a key role in the interaction between CCKBR, D5R, and water-sodium metabolism by improving protein transfer from the cytoplasm to cell membrane.关键词
β-tubulin/D5R/CCKBR/蛋白转运Key words
β-tubulin/D5R/CCKBR/protein transfer分类
医药卫生引用本文复制引用
刘星,刘云鹏,付慧,刘雪,姜晓亮,杨志伟..β-tubulin 在肾脏D5R 与CCKBR 相互调节中的重要作用[J].中国比较医学杂志,2018,28(5):39-45,7.基金项目
国家自然科学基金面上项目(编号:81670387) （编号:81670387）
国家自然科学基金青年项目(编号:81600334) （编号:81600334）
中国医学科学院医学与健康科技创新工程重大协同创新项目(编号:2016-I2M-1-016) （编号:2016-I2M-1-016）
生命科学学会联合体"青年人才托举工程". （）
