中国比较医学杂志2018，Vol.28Issue(5)：46-52,7.DOI:10.3969/j.issn.1671-7856.2018.05.009
Sestrin2 在吸烟诱导的肺泡Ⅱ型上皮细胞损伤中的作用机制
Protective effect and mechanism of Sestrin2 in the development of pulmonary alveolar type II epithelial cell injury induced by cigarette smoking
摘要
Abstract
Objective To explore the role of Sestrin2 in pulmonary alveolar type II epithelial cell injury induced by cigarette smoking and its mechanism of action. Methods The cell injury model was induced by cigarette smoke extract (CSE)in the human pulmonary alveolar type II epithelial A549 cells. The generation of ROS was detected by DCFDA fluorescence probe. The levels of inflammatory factors TNF-α and IL-8 were determined by ELISA, and the expression of Sestrin2 and the peroxiredoxin,Prx-SO2/3H,was detected by Western blot. In addition,all the events were also measured in the A549 cells which were transfected with Sestrin2 siRNA and treated with azithromycin. Results After the CSE treatment,the expression of Sestrin2 in the A549 cells was decreased, the expression of Prx-SO2/3H was increased, the ROS production,secretion of cytokines TNF-α and IL-8 were increased(P < 0.05). These changes were partly reducedby azithromycin, indicating that azithromycin significantly relieved CSE-induced oxidative stress and inflammatory injury.Silencing of Sestrin2 in the A549 cells result ed in an increase of Prx-SO2/3 H expression, ROS production and the secretionof the cytokines TNF-α and IL-8. However, oxidative stress and inflammatory injury were not alleviated with the addition ofazithromycin in the Sestrin2 siRNA silencing A549 cells. Conclusions Sestrin2 plays an protective role in the pulmonaryalveolar type II epithelial cell injury induced by cigarette smoking through negatively regulating the level of intracellularROS via catalyzing the reduction of the hyperoxidized peroxiredoxin Prx-SO2/3 H.关键词
肺泡上皮细胞损伤/慢性阻塞性肺疾病/香烟烟雾/Sestrin2/活性氧Key words
pulmonary alveolar epithelial cell injury/chronic obstructive pulmonary disease/COPD/cigarette smoke/Sestrin2/reactive oxygen species/ROS分类
医药卫生引用本文复制引用
姜晓亮,刘雪,刘云鹏,付慧,刘星,杨志伟..Sestrin2 在吸烟诱导的肺泡Ⅱ型上皮细胞损伤中的作用机制[J].中国比较医学杂志,2018,28(5):46-52,7.基金项目
国家自然科学基金面上项目(编号:81600387) （编号:81600387）
国家自然科学基金青年项目(编号:81600334) （编号:81600334）
中国医学科学院医学与健康科技创新工程重大协同创新项目(编号:2016-I2M-1-016) （编号:2016-I2M-1-016）
生命科学学会联合体"青年人才托举工程". （）
