中国比较医学杂志2018，Vol.28Issue(5)：65-69,5.DOI:10.3969/j.issn.1671-7856.2018.05.012
氯硝柳胺乙醇胺盐对大鼠冠状动脉的舒张作用及机制研究
Vasorelaxant effect and mechanism of niclosamide ethanolamine on rat coronary artery
摘要
Abstract
Objective To investigate the vasorelaxant effect and possible mechanism of niclosamide ethanolamine (NEN)on isolated rat coronary artery(RCA). Methods Wire myograph was used to record myogenic tone of vessels. The vasorelaxant effect of NEN was studied in RCA precontracted with either KCl or U46619. Study of related inhibitors was performed to investigate possible involvement of potassium channels and the mitogen-activated protein kinase(MAPK) signaling pathway in vasorelaxation. The effect of NEN on Ca2+mobilization was determined by observing vasoconstrictor-induced contractions in tissue solution deprived of Ca2+followed by Ca2+restoration. Results NEN(0.5-3.0 μmol/L) relaxed RCA precontracted with KCl or U46619 in a concentration-dependent manner. MAPK inhibitors(PD98059 and SB239063)reduced the relaxant effect of NEN,while the potassium-channel blockers(tetraethylamine,4-aminopyridine, BaCl2,and glibenclamide)did not significantly affect relaxation. NEN specifically inhibited the contraction component dependent on extracellular Ca2+influx in vessels stimulated with KCl and U46619, with a negligible effect on the component dependent on intracellular Ca2+release. Conclusions NEN exhibits vasodilator properties in RCA. Inhibition of extracellular Ca2+influx and activation of the MAPK signaling pathway may be involved in NEN-induced RCA vasorelaxation.关键词
氯硝柳胺乙醇胺盐/血管舒张/冠状动脉/MAPK信号通路/Ca2+Key words
niclosamide ethanolamine/NEN/vasorelaxtion/coronary artery/MAPK signaling pathway/Ca2+分类
医药卫生引用本文复制引用
宋奇颖,郭鹏美,董丽娜,董苗苗,刘宇..氯硝柳胺乙醇胺盐对大鼠冠状动脉的舒张作用及机制研究[J].中国比较医学杂志,2018,28(5):65-69,5.基金项目
国家自然科学基金资助项目(编号:81603111) （编号:81603111）
山西省"1331工程"重点学科建设计划支持学科. （）
