中国比较医学杂志2018，Vol.28Issue(5)：75-80,6.DOI:10.3969/j.issn.1671-7856.2018.05.014
SIV 感染猴外周血CD14+ 单核细胞CD169分子表达的变化
Changes in the expression of CD169 on peripheral blood CD14 +monocytes of rhesus monkeys after SIV infection
摘要
Abstract
Objective To investigate the changes of CD169 expression on the surface of peripheral blood monocytes and different subsets of monocytes in normal rhesus monkeys after SIVmac239 infection and the possible reasons.Methods Normal rhesus monkeys were infected with SIVmac239 through intravenous injection, and changes in the percentage of peripheral blood monocytes and the expression of CD169 before and after SIVmac239 infection were detected by flow cytometry. The peripheral blood CD14 +monocytes of normal rhesus monkeys sorted by flow cytometry were directly infected by SIVmac239 and stimulated by different cytokines,and changes in the expression of CD169 on the cell surface and the cytokine IFN-α were detected by flow cytometry. Results After SIVmac239 infection, the percentage of CD14 +monocytes of the normal rhesus monkeys was decreased and the expression of CD169 on their surface was increased. Meanwhile,the expression of CD169 on the surface of different subsets of peripheral blood monocytes was significantly increased,and the expression of CD169 on the CD14 +CD16 + +monocytes was increased more obviously. CD169 was not expressed on the surface of peripheral blood CD14 +monocytes of the normal rhesus monkeys after stimulated by the cytokines M-CSF, IL-4 and IL-13. However, CD169 was highly expressed after the monocytes were stimulated by the cytokine IFN-α. The expression of CD169 on the surface of CD14 +monocytes and the intracellular cytokine IFN-α was not significantly changed after the monocytes were directly infected with SIVmac239. Conclusions SIVmac239 infection can lead to the increase of CD169 expression on the surface of peripheral blood monocytes in rhesus monkeys. Its expression is not associated with the direct infection of virus,but is related to the cytokine IFN-α secreted by other cells of the monkeys in vivo.关键词
猴免疫缺陷病毒/单核细胞/IFN-α/CD169Key words
simian immunodeficiency virus/SIV/monocytes/IFN-α/CD169分类
医药卫生引用本文复制引用
李想,薛婧,陈霆,丛喆,魏强..SIV 感染猴外周血CD14+ 单核细胞CD169分子表达的变化[J].中国比较医学杂志,2018,28(5):75-80,6.基金项目
中国医学科学院医学与健康科技创新工程重大协同创新项目(编号:2017-I2M-1-014) （编号:2017-I2M-1-014）
科技部重大专项(编号:2017ZX10304402). （编号:2017ZX10304402）
