中国比较医学杂志2018，Vol.28Issue(5)：100-104,5.DOI:10.3969/j.issn.1671-7856.2018.05.018
树鼩副粘病毒(TPMV)PCR 方法的建立及初步应用
Establishment and application of a PCR detection method for Tupaia (tree shrew)paramyxovirus(TPMV)
王淑菁 1付瑞 1李晓波 1王吉 1贺争鸣 1岳秉飞1
作者信息
- 1. 中国食品药品检定研究院实验动物资源研究所,国家实验动物质量检测中心,北京 100050
- 折叠
摘要
Abstract
Objective To establish and preliminarily apply an effective PCR assay for detection of Tupaia(tree shrew)paramyxovirus(TPMV). Methods Using TPMV genomic DNA from NCBI GenBank, bases 8231 to 8720 were synthesized and inserted into a plasmid as a positive standard. One primer pair was designed based on this sequence. In total,60 respiratory swabs and 12 lung tissues from the tree shrews were tested in this PCR assay. Results A PCR method for detection of TPMV was successfully established,with high specificity and sensitivity of 11.5 × 10 -5μg/mL. PCR result testing 60 respiratory swabs and 12 lung tissues were negative. Conclusions PCR for detecting TPMV has good specificity and high sensitivity and can be used for conventional tree shrew paramyxovirus detection.关键词
树鼩/副粘病毒/PCR/检测Key words
Tupaia(tree shrew)/paramyxovirus/PCR/detection分类
医药卫生引用本文复制引用
王淑菁,付瑞,李晓波,王吉,贺争鸣,岳秉飞..树鼩副粘病毒(TPMV)PCR 方法的建立及初步应用[J].中国比较医学杂志,2018,28(5):100-104,5.
