中国比较医学杂志2018，Vol.28Issue(5)：105-108,4.DOI:10.3969/j.issn.1671-7856.2018.05.019
C57BL/6 小鼠尾静脉四种穿刺方法的比较
Comparison of different puncture methods in C57BL/6 mouse tail veins
温军业 1范晓燕 2何东伟 3张万星 1黄书静 1张宇龙4
作者信息
- 1. 河北省人民医院普外三科,石家庄 050000
- 2. 河北省人民医院肿瘤科,石家庄 050000
- 3. 河北医科大学第四医院,石家庄 050000
- 4. 石家庄市第一医院外三科,石家庄 050000
- 折叠
摘要
Abstract
Objective Four different methods were examined to identify a safer and more reliable method for tail vein punctures in C57BL/6 mice. Methods In total,320 mice were randomly divided into four groups: a blank group, incandescent lamp baking method group,three-line method group,and combined method group. Blood samples were taken from the left or right peripheral vein of puncture mice. Puncture success rate of each group was recorded. SPSS 13.0 software was used to compare statistical difference among groups. Results Compared with the blank group,success rates of the other three methods were significantly higher(P < 0.001). Further, the three-line method was better than the incandescent lamp baking method(P< 0.001). The success rate of the combined method was significantly higher than the three-line and incandescent lamp baking methods(P< 0.001). Conclusions The combined method greatly improved the success rate of tail vein punctures in C57BL/6 mice. This method is more reliable and should be more widely used in the future.关键词
尾静脉注射/三头线法/联合应用法Key words
tail vein injection/three-line method/combined method分类
医药卫生引用本文复制引用
温军业,范晓燕,何东伟,张万星,黄书静,张宇龙..C57BL/6 小鼠尾静脉四种穿刺方法的比较[J].中国比较医学杂志,2018,28(5):105-108,4.
