中国比较医学杂志2018，Vol.28Issue(5)：114-118,5.DOI:10.3969/j.issn.1671-7856.2018.05.021
基于树突状细胞激活建立皮肤致敏的体外方法
In vitro method for skin sensitization based on dendritic cell activation
摘要
Abstract
Allergy contact dermatitis is a type IV delayed type hypersensitivity that is induced by exogenous compounds and involves many cell types. Traditional animal testing uses guinea pigs or mice as a model. With progress of the adverse outcome pathway(AOP)on skin sensitization, a concept for development of alternative methods based on a molecular initiating event and key events is provided. Dendritic cell(DC)activation plays a key role in the AOP. Many alternative methods have been developed,with several methods validated and accepted as guidance for assays. This paper examines DC screening, characteristics of test parameters, and limitations and applicability of DC-derived methods. Progress on interactions between DCs and other cells, co-culture systems, and the human body-on-a-chip will also be introduced. Altogether,this paper will provide information for optimization of in vitro alternative methods for sensitization detection.关键词
皮肤致敏/树突状细胞/替代方法/有害结局通路Key words
skin sensitization/dendritic cells/DCs/alternative methods/adverse outcome pathway/AOP分类
医药卫生引用本文复制引用
陈健,柯逸晖,陈彧,谈伟君,程树军..基于树突状细胞激活建立皮肤致敏的体外方法[J].中国比较医学杂志,2018,28(5):114-118,5.基金项目
广州经济技术开发区国际科技合作项目(编号:2017GH11). （编号:2017GH11）
