中国药理学与毒理学杂志2018，Vol.32Issue(2)：99-104,6.DOI:10.3867/j.issn.1000-3002.2018.02.003
丹参总酚酸诱导PC12细胞分化机制
Mechanism of PC12 cell differentiation induced by total salvianolic acid
摘要
Abstract
OBJECTIVE To study the differentiation of PC12 cells induced by total salvianolic acid (Tsa) and the mechanism. METHODS MTT assay was used to detect the effect of Tsa 0.01, 0.1 and 1.0 μg·L-1on proliferation of PC12 cells and on the cells damaged by oxygen and glucose deprivation (OGD).The number of projections of PC12 cells was statistically analyzed.Western blotting was applied to detect the levels of microtubule-associated protein2 (MAP-2), extracellular signal-regulated kinase1/2 (ERK1/2), phosphorylated ERK1/2(p-ERK1/2), mitogen-activated protein kinase kinase1/2(MEK1/2) and p-MEK1/2 proteins.MEK inhibitor U0126 was examined for its effect on expressions of p-ERK1/2 and ERK1/2 protein in PC12 cells induced by Tsa 1.0 μg·L-1.RESULTS Compared with normal control group, Tsa 1.0 μg·L-1could promote PC12 cell proliferation, and the survival rate was increased by 90%, but the survival rate of PC12 cells was not affected by Tsa 0.01 or 0.1 μg·L-1. Compared with OGD injured group,PC12 cells injured by OGD could be repaired by Tsa 0.1 or 1.0 μg·L-1,and the survival rate was increased to (47.7±1.8)% and (63.2±13.0)%, respectively (P<0.05, P<0.01). Compared with normal control group,Tsa 0.01,0.1 and 1.0 μg·L-1could promote the growth of PC12 cell projections (P<0.01). Western blotting results showed that Tsa could promote the expressions of MAP-2, p-ERK1/2 and p-MEK1/2 proteins, and this effect could be blocked by U0126 inhibitor (P<0.01). CONCLUSION Tsa can induce the proliferation and differentiation of PC12 cells, the mechanism of which is possibly the activation of p-MEK and p-ERK1/2.关键词
丹参总酚酸/细胞分化/细胞外信号调节激酶Key words
total salvianolic acid/differentiation/extracellular signal-regulated kinase分类
医药卫生引用本文复制引用
沈杨,张琦,赵佳奇,田雅娟,李钦青,楚世峰,贺文彬..丹参总酚酸诱导PC12细胞分化机制[J].中国药理学与毒理学杂志,2018,32(2):99-104,6.基金项目
国家自然科学基金项目(81273629) （81273629）
国家自然科学基金项目(81473375) （81473375）
国家自然科学基金项目(81530099)山西省回国留学人员科研项目(2013-134) （81530099）
山西省留学回国人员科技活动择优资助项目 （）
山西省高等学校创新人才支持计划 （）
中国博士后科学基金一等资助项目(2013M540066).The project supported by National Natural Science Foundation of China(81273629) （2013M540066）
National Natural Science Foundation of China(81473375) （81473375）
National Natural Science Foundation of China(81530099) （81530099）
Research Project Supported by Shanxi Scholarship Council of China(2013-134) （2013-134）
Program for the Innovative Talents of Higher Learning Institutions of Shanxi （）
Fund Program for the Scientific Activities of Selected Returned Overseas Professionals in Shanxi Province （）
and China Postdoctoral Science Foundation(2013M540066) （2013M540066）
