中国药理学与毒理学杂志2018，Vol.32Issue(2)：119-124,6.DOI:10.3867/j.issn.1000-3002.2018.02.006
ZnO纳米颗粒水凝胶高分子材料的合成及梭曼体内外染毒洗消性能的评价
Synthesis of hydrogel polymer coated ZnO nanoparticles and their decontaminating effect against soman in vitro
摘要
Abstract
OBJECTIVE To evaluate the decontamination capability of hydrogel polymer coated ZnO nanoparticles (ZnO NP-gel) against soman. METHODS ZnO NP was synthetized using chemical precipitation method and modified with 4-pentenoic acid,and then polymerized with comonomers to obtain ZnO NP-gel. The transmission electron microscope (TEM), scanning electron microscope (SEM) and particle size instrument were used to observe the internal structure,micromorphology,particle size and zeta potential of these materials. An infrared spectroscope (IR) was used to analyze their chemical bond structure,while X-ray diffraction (XRD) was used to analyze the diffraction pattern.The content of soman was determined by benzidine chromogenic reaction. ZnO NP(1 g·L-1), ZnO NP-gel (1 g·L-1) and distilled water were mixed with soman(52.2 mg·L-1),stood for 30 min,and then filtered before filtrate was subcutaneously injected into mice (40 μL·g-1) to observe the symptoms of poisoning and death. RESULTS SEM and TEM showed that ZnO NP-gel had a block structure, the zeta potential of which was (-7.89 ± 0.04) mV. The results of IR indlicated that ZnO NP-gel had stronger absorption peaks at 754 and 618 cm-1, and XRD revealed that these materials had a sharp peak at 2θ=8.06738°. The decontamination efficiency of ZnO NP-gel was higher than that of ZnO NP group at the same concen?tration (n=3, P<0.05), and the time for decontamination of 50% soman was shortened by four times. The mice were injected subcutaneously with the soman solution treated with ZnO NP-gel, which caused no convulsion or death. CONCLUSION ZnO NP-gel can perform the double function of fast adsorption and catalysis of soman,and the decontamination ability of which could be improved through polymer modification.关键词
神经性毒剂/氧化锌/金属氧化物/水凝胶/纳米颗粒/洗消/梭曼Key words
nerve agents/zinc oxide/metallic oxide/hydrogel/nanoparticles/decontamination/soman分类
医药卫生引用本文复制引用
王甲朋,张有志,杨军,孟凡荣,范丽雪,李万华,隋昕,骆媛,田萌,全东琴,王永安..ZnO纳米颗粒水凝胶高分子材料的合成及梭曼体内外染毒洗消性能的评价[J].中国药理学与毒理学杂志,2018,32(2):119-124,6.基金项目
国家自然科学基金项目(81402847).The project supported by National Natural Science Foundation of China(81402847) （81402847）
