中国中医药信息杂志2018，Vol.25Issue(6)：61-65,5.DOI:10.3969/j.issn.1005-5304.2018.06.015
健脾消癌方对人结肠癌HCT116细胞周期、凋亡及Wnt/β-catenin信号通路相关因子的影响
Effects of Jianpi Xiaoai Prescription on Cell Cycle and Apoptosis of Human Colon Cancer HCT116 Cells and Wnt/β-catenin Signaling Pathway Related Proteins
摘要
Abstract
Objective To study the effects of Jianpi Xiaoai Prescription on cell cycle and apoptosis of human colon cancer HCT116 cells and related factors of Wnt/β-catenin signaling pathway; To investigate its mechanisms of anti-metastasis and recurrence of colorectal cancer. Methods The logarithmic growth phase HCT116 cells were divided into blank group, saline group, 5-Fu group, and Jianpi Xiaoai Prescription low-, medium-, and high-dose groups. After intervention for 48 h, the cells were harvested, and the cell cycle and apoptosis were detected by flow cytometry. The protein expression of β-catenin in the nucleus was detected by Western blot, and the expression of c-myc and cyclinD1 mRNA was detected by PCR. Results Compared with the blank group and saline group, the ratio of HCT116 cells apoptosis of Jianpi Xiaoai Prescription low-, medium-, and high-dose groups increased; the proportion of cells in phase G1 increased and the proportion of S cells decreased; the expression of β-catenin protein in the nucleus and the expression of c-myc,cyclinD1 mRNA decreased,especially in the Jianpi Xiaoai Prescription high-dose group,with statistical significance(P<0.05,P<0.01).Conclusion Jianpi Xiaoai Prescription can promote apoptosis of human colon cancer HCT116 cells and block the cell cycle, and its mechanism may be related to regulating Wnt/β-catenin signaling pathway.关键词
健脾消癌方/大肠癌/Wnt/β-catenin信号通路/细胞凋亡/细胞周期Key words
Jianpi Xiaoai Prescription/colorectal cancer/Wnt/β-catenin signaling pathway/apoptosis/cell cycle分类
医药卫生引用本文复制引用
杨晓,唐蔚,蒋益兰,陶子豪,刘科,宋程..健脾消癌方对人结肠癌HCT116细胞周期、凋亡及Wnt/β-catenin信号通路相关因子的影响[J].中国中医药信息杂志,2018,25(6):61-65,5.基金项目
湖南省中医药科技计划项目(201624) （201624）
