中医药学报2018，Vol.46Issue(3)：14-21,8.DOI:10.19664/j.cnki.1002-2392.180072
丹参多化学成分的UPLC-LTQ Orbitrap MS快速表征解析
Quick Feature Analyzation of Chemical Constituents of Radix et Rhizoma Salviae Miltiorrhizae by UPLC-LTQ Orbitrap MS
摘要
Abstract
Objective:To establish a quick identification method of UPLC-LTQ Orbitrap MS ( ultra-high performance liquid chromatography-linear trap/orbitrap high resolution mass spectrometry) for the identification of chemical constit-uents in Danshen( Radix et Rhizoma Salviae Miltiorrhizae) . Methods:The negative and positive ion model was used for Orbitrap MS scanning, the components were identified by high resolution data and the secondary mass spectrum informa-tion. Results:Debris features and mass spectrum cracking rule were concluded in the standard substance of tanshinone IIA and Dan phenolic acid B. 70 components including diterpenes, phenolic acids, and quinones were discovered from extract of Radix et Rhizoma Salviae Miltiorrhizae. Conclusion: This method could provide rapid qualitative analysis for the chemical components of Radix et Rhizoma Salviae Miltiorrhizae, thus to provide technical support for the quality con-trol of it and its pharmacodynamics research. It can also provide reference for comprehensive characterization of complex chemical constituents of Chinese herbs.关键词
超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱(UPLC-LTQOrbitrapMS)/丹参/萜类/酚酸Key words
UPLC-LTQ Orbitrap MS/Danshen( Radix et Rhizoma Salviae Miltiorrhizae)/Terpenoids/Phenolic acid分类
医药卫生引用本文复制引用
张驰,王学勇,张彩娟,邱敏懿,晋小雁,邵远洋,刘莉,王敏,卢彦冰,王春国..丹参多化学成分的UPLC-LTQ Orbitrap MS快速表征解析[J].中医药学报,2018,46(3):14-21,8.基金项目
国家自然科学基金项目(81773841) （81773841）
贵州省中药现代化科技产业研究开发专项(黔科合ZY字〔2013〕3004号) （黔科合ZY字〔2013〕3004号）
北京中医药大学基本科研业务项目青年教师基金(2017-JYB-JS022) （2017-JYB-JS022）
