中医药学报2018，Vol.46Issue(3)：21-23,3.DOI:10.19664/j.cnki.1002-2392.180073
HPLC法测定槟榔中槟榔碱和槟榔次碱的含量
Determination of Arecoline and Arecaidine in Semen Arecae by HPLC
摘要
Abstract
Objective:To establish an determination method for arecoline and arecaidine in Binglang( Semen Arecae) by HPLC. Methods:Nucleosil SA cation-exchange column(4. 6 mm × 250 mm, 5μm) was used with methanol-water-phosphoyic acid (60:40:0. 3, pH 3. 8) as the mobile phase at the flow rate of 1. 0 mL·min-1. The detection wave length was 212 nm and the column temperature was 30℃. Results: Arecoline and arecaidine were well isolated from other substances. For arecoline, there was a good linearity in the range of 2. 02～32. 30 μg·ml-1(R2 =0. 9995) and the average recovery rate was 97. 2％ with RSD as 2. 4％ (n=9). For arecaidine, there was a good linearity in the range of 0. 85～13. 55 μg·ml-1(R2 =0. 9993), and the average recovery rate was 103. 9％ with RSD as 2. 6％(n=9 ) . Conclusion:The determination method is specific, accurate and repeatable, which can be used for determing areco-line and arecaidine in Semen Arecae.关键词
槟榔/槟榔碱/槟榔次碱/高效液相色谱法Key words
Binglang( Semen Arecae)/Arecoline/Arecaidine/HPLC分类
医药卫生引用本文复制引用
李春燕,张学敏,岳璐,闫福林..HPLC法测定槟榔中槟榔碱和槟榔次碱的含量[J].中医药学报,2018,46(3):21-23,3.基金项目
河南省高等学校重点科研项目(17A360026,17A350002) （17A360026,17A350002）
河南省科技攻关计划(172102310326) （172102310326）
