中医药学报2018，Vol.46Issue(3)：24-27,4.DOI:10.19664/j.cnki.1002-2392.180074
川楝子炒制前后对大鼠在体肠吸收特性的影响
Effect of Toosendan Fructus on Intestinal Absorption in Rats Before and After Stir-Frying Process
摘要
Abstract
Objective:To investigate the difference of intestinal absorption for toosendanin extracted from Toosendan Fruc-tus before and after stir-frying process. Methods:The intestinal perfusion in vivo model was used to determine the con-centration of toosendanin by HPLC. Results:Absorption rate constant( Ka) of toosendanin in duodenum, jejunum, ile-um, and colon were in the range of (746. 30 ± 40. 31/s～347. 84 ± 111. 67/s) in raw extract, (473. 77 ± 22. 05/s～243 . 33 ± 19 . 58/s ) in extract of stir - frying Toosendan Fructus ( same amount of crude herb ) , and ( 724 . 93 ± 57.59/s～290.78 ±41.66/s) in extract of stir-frying Toosendan Fructus(same amount of toosendanin). In terms of permeability coefficient(Papp) in each segment of the intestine, it was (83. 66 ± 7. 00 cm/s～58. 33 ± 16. 49 cm/s) in raw extract, (43. 57 ± 8. 63 cm/s～33. 89 ± 1. 55 cm/s) in extract of stir-frying Toosendan Fructus( same amount to crude herb), and (81.67 ±13.00 cm/s～51.86 ±5.78 cm/s) in extract of stir-frying Toosendan Fructus(same a-mount of toosendanin) . Conclusions:The index component of Toosendan Fructus, toosendanin, has good intestinal ab-sorption. There was difference in intestinal absorption of toosendanin between raw Toosendan Fructus and stir-frying Toosendan Fructus with the same amount of raw herb;there was no significant difference in intestinal absorption with the same amout of toosendanin. The results showed after stir-frying process, the content of toosendanin had changed, thus to influence its intestinal absorption. This study initially revealed the attenuation mechanism of stir-frying process for Toosendan Fructus.关键词
炒制/川楝子提取物/在体单向肠灌流/肠吸收Key words
Stir -frying process/Extract of Toosendan Fructus/Single -pass intestinal perfusion in vivo/Intestinal absorption分类
医药卫生引用本文复制引用
张景珍,王英姿,李文华,张春泥,王思雨,聂瑞杰,孙振阳..川楝子炒制前后对大鼠在体肠吸收特性的影响[J].中医药学报,2018,46(3):24-27,4.基金项目
国家科技基础性工作专项资助项目(2014FY111100-3) （2014FY111100-3）
北京中医药大学校级课题东直门医院"111"协同创新院际合作项目(2016-DZM111-ZY006) （2016-DZM111-ZY006）
北京中医药大学在读研究生项目(2017-JYB-XS-064) （2017-JYB-XS-064）
