中医药学报2018，Vol.46Issue(3)：31-35,5.DOI:10.19664/j.cnki.1002-2392.180076
麻黄定喘汤和鹿茸大补汤的急性毒性研究
Acute Toxicity of Mahuang Dingchuan Decoction and Lurong Dabu Decoction
摘要
Abstract
Objective:To study the acute toxicity of Mahuang Dingchuan Decoction and Lurong Dabu Decoction in mice to evaluate their safety. Methods:50 Kunming mice were given medications through gavage administration. LD50 was too large to caculate in the preliminary experiment. 100 times dose of clinical adult dosage was applied to mice, i. e. for Mahuang Dingchuan Decoction, it was 23000 mg·kg-1; for Lurong Dabu Decoction, it was 26000 mg·kg-1; the control group was given normal saline with dose of 10 ml·kg-1 . Body status, mental state, body mass, and food intake were observed in mice for continuous 7 days. After 7 days of observation, the mice were sacrificed by cervical dislocation method. Samples of brain, liver, spleen, lung, and kidney were weighed to calculate organ coefficient. The tissues of mice were examined by biochemical detection to measure the activities of glutamic - oxalacetic transaminase and glutamic-pyruvic transaminase. The data were compared in the three groups. Results: The acute toxicity test showed no symptoms of poisoning, no animal death, and the mice showed normal appearance. There were no significant differences among three groups ( P >0 . 05 ) . Conclusion: Mahuang Dingchuan Decoction and Lurong Dabu Decoction have high safety, which can be both applied in clinic with right amount.关键词
麻黄定喘汤/鹿茸大补汤/急性毒性/昆明鼠种/半数致死量Key words
Mahuang Dingchuan Decoction/Lurong Dabu Decoction/Acute toxicity/Kuming mice/LD50分类
医药卫生引用本文复制引用
李聪,郑明昱,许妍姬..麻黄定喘汤和鹿茸大补汤的急性毒性研究[J].中医药学报,2018,46(3):31-35,5.基金项目
国家自然科学基金项目(81560810) （81560810）
吉林省教育厅"十三五"科学技术研究项目(吉教科合字〔2016〕第279号) （吉教科合字〔2016〕第279号）
延边大学医药健康产品研发项目(延大科合字〔2016〕6号) （延大科合字〔2016〕6号）
