中医药学报2018，Vol.46Issue(3)：39-42,4.DOI:10.19664/j.cnki.1002-2392.180078
三氧化二砷对慢性粒细胞白血病Hedgehog信号转导通路分子蛋白表达的影响
Effect of Arsenic Trioxide on Molecular Gene Expression of Hedgehog Signaling Pathway of Chronic Myelogenous Leukemia
摘要
Abstract
Objective:Cell line K562 of human chronic myelogenous leukemia( CML) was used as the model to investi-gate whether arsenic trioxide(ATO) can induce apoptosis of CML cells, and to explore whether ATO can inhibit the relative molecule expression of CML Hedgehog( Hh) signal transduction pathway, so that providing the theoretical basis for applying ATO to CML with Hh activation. Methods:Different concentrations of ATO was applied in K562 cells for 48 hours, then flow cytometry was used to detect the apoptosis-inducing effect of ATO, and then Western blot ( WB) was used to detect the changes of Hh signal transduction molecule Gli2, Gli1, SMO and Ptch2 at protein levels. Results:Af-ter ATO intervention, K562 cells showed dose-dependent apoptosis effects, the protein expression of Gli2 and Ptch2 were significantly affected, and the difference was statistically significant. Conclusion:For CML cells, ATO may inhibit Hh signaling pathway by Gli2 protein target . This research provides a theoretical basis for expanding ATO application in CML.关键词
慢性粒细胞白血病/三氧化二砷/Hedgehog信号转导通路/Gli1核转录因子/Gli2核转录因子Key words
Chronic myeloid leukemia/Arsenic trioxide/Signal transduction pathway of Hedgehog/Nuclear factor Gli1/Nuclear factor Gli2分类
医药卫生引用本文复制引用
马咏歌,宋鹏,叶向梅,赵辉,刘志宇,张丽坤,刚宏林,杨东光..三氧化二砷对慢性粒细胞白血病Hedgehog信号转导通路分子蛋白表达的影响[J].中医药学报,2018,46(3):39-42,4.基金项目
国家自然科学基金面上项目(81500117) （81500117）
黑龙江省自然科学基金青年基金项目(H2015058) （H2015058）
黑龙江省博士后科研启动资金项目(LBH-Q14129) （LBH-Q14129）
