长春中医药大学学报2018，Vol.34Issue(3)：415-418,4.DOI:10.13463/j.cnki.cczyy.2018.03.003
参附芎泽方对心肌梗死后心力衰竭模型大鼠心肌能量代谢的影响
Effects of Shenfu Xiongze decoction on myocardial energy metabolism in heart failure rat after myocardial infarction
摘要
Abstract
Objective To study the effects of Shenfu Xiongze decoction on the myocardial energy metabolism in heart failure rats after myocardial infarction.Methods SD rats were divided into sham operation group, model group,Shenfu Xiongze decoction group and trimetazidine group. Adopted to left anterior descending coronary artery ligation is establish the model of heart failure after myocardial infarction. The sham operation group and model group were given pure water,Shenfu Xiongze decoction group and trimetazidine group were given Shenfu Xiongze decoction (containing crude drug 8.4g/kg) and trimetazidine (containing 6.3g/kg) corresponding to the volume of liquid. Fill the stomach with 20 mL/kg, 1 times a day for 28 days. At 1 hour after the last administration, blood was collected from abdominal aorta, blood was centrifuged, and serum was collected. The level of serum CK,CK-MB,FFA was detected by ELISA method. The expression of AMPK,GLUT4 in myocardial tissue was detected by Western blot. The pathological changes of myocardium were observed by HE staining.Results Compared with the sham operation group, the levels of serum CK, CK-MB and the myocardial tissue expression of AMPK and GLUT4 in the model group decreased, the content of FFA increased (P<0.05). Compared with the model group, the Shenfu Xiongze decoction group and trimetazidine group, the serum CK, CK-MB content elevated, FFA content decreased, the expression of AMPK and GLUT4 protein in myocardial tissue were increased. (P<0.05). Compared with trimetazidine group, the Shenfu Xiongze decoction group, the serum CK-MB content was increased, the content of FFA in serum and the expression of AMPK protein in myocardial tissue were decreased (P<0.05). HE staining results: the morphological of myocardial tissue in heart failure rats after myocardial infarction have obvious changes, myocardial necrosis, focal necrosis of muscle fiber in the dissolution, myocardial fiber hypertrophy, muscle fibers with local rupture, atrophy and degeneration, myocardial interstitial edema and inflammatory cell infiltration. trimetazidine and Shenfu Xiongze decoction group can significantly relieve the morphological of myocardial tissue changes. Conclusion Shenfu Xiongze decoction can increase serum CK, CK-MB content, reduce the content of serum FFA, By regulating the expression of AMPK and GLUT4 proteins in myocardium, reduce myocardial tissue injury, improve heart failure.关键词
参附芎泽方/心肌梗死/心力衰竭/心肌能量代谢Key words
Shenfu Xiongze decoction/myocardial infarction/heart failure/myocardial energy metabolism分类
医药卫生引用本文复制引用
钟伟,于远望,张淑珍,李彩红,齐婧,尚俊平,王永刚..参附芎泽方对心肌梗死后心力衰竭模型大鼠心肌能量代谢的影响[J].长春中医药大学学报,2018,34(3):415-418,4.基金项目
陕西省科技厅社会发展科技攻关项目(2016SF-348) （2016SF-348）
陕西省教育厅科研计划项目(16JK1205) （16JK1205）
陕西省教育厅重点实验室项目(17JS028) （17JS028）
陕西省中医药管理局项目(LCPT089) （LCPT089）
陕西中医药大学科研基金项目(2015QN26) （2015QN26）
国家中医药管理局重点专科心血管病科建设项目. （）
