长春中医药大学学报2018，Vol.34Issue(3)：422-425,4.DOI:10.13463/j.cnki.cczyy.2018.03.005
针刺预处理对大鼠缺血心肌线粒体通透孔开放的调节
Effect of acupuncture pretreatment on opening of mitochondrial permeability in rats with ischemic myocardium
摘要
Abstract
Objective To investigate molecular mechanism by study of Xinshu electrical acupuncture preconditioning on the opening of mitochondrial permeability in rats with ischemic myocardium.Methods Rats were randomly divided into blank group, model group, acupuncture Xinshu preconditioning group (experimental group) and ischemic preconditioning group (control group). MIRI model was established by ligation of left anterior descending coronary artery in living rat heart. Blank group: fixed 30 min without acupuncture once a day, continuous 15 days.Model group: same blank group before modeling and control group.In the experimental group, acupuncturewas started at 15 d before making the film, 1 times /d, and 30min/ times, with a total of 15 d. In the control group, the blood flow was blocked for 5 minutes before coronary artery ligation and reperfusion for 5 minutes, followed by 30 min ischemic preconditioning for 3 consecutive times.Immediately after the successful establishment of myocardial ischemia model, 24, 48 hours.The open degree of mPTP and its effect on the expression of Bal-2 Caspase-3 in cardiomyocytes were detected.Results Model group dyed myocardial mitochondria green fluorescent compared with the blank group was reduced obviously, the experiment result shows that the mitochondrial membrane permeability transition pore activity of model group was significantly reduced (P<0.01). Compared with the model group, LSCM observation showed that the myocardial mitochondrial fluorescence intensity was significantly decreased in the control group and the experimental group, but there was no significant difference between the control group and the immediate experimental group (P>0.05). Test 24 h, 48 h group mitochondria fluorescence intensity have a certain enhancement (P<0.05 orP<0.01) observed compared with control group and test 0h group. Experiment data shows that test and control group both can reduce MIRI.Test group can down-regulate Caspase-3 and up-regulate Bal-2.Conclusion The protective effect of electroacupuncture pretreatment on Xinshu at different time after myocardial ischemia and the difference of therapeutic effect was enhanced with the change of the time to peak - weakening trend. Electroacupuncture Xinshu pretreatment has protective effects on myocardial Miri, its mechanism may be through the regulation of MIRI during myocardial MPTP open, reduce mitochondrial apoptosis, reduce myocardial injury.关键词
针灸推拿学/针刺预处理/缺血预处理/激光共聚焦显微镜/线粒体通透性转换孔Key words
acupuncture and massage/acupuncture preconditioning/ischemic preconditioning/the laser confocal microscope/mitochondrial permeability transition pore分类
医药卫生引用本文复制引用
宋春华,金艳红,闫珺,亢连茹,丁续,王保叶,付嘉明,徐红飞,公维志,董勤建,李晓艳,郭丹丹..针刺预处理对大鼠缺血心肌线粒体通透孔开放的调节[J].长春中医药大学学报,2018,34(3):422-425,4.基金项目
黑龙江省自然科学基金面上项目"针灸治疗疑难病及对神经-内分泌-免疫网络系统的调节研究"(H2015035). （H2015035）
