发光学报2018，Vol.39Issue(5)：621-626,6.DOI:10.3788/fgxb20183905.0621
基于萘酰腙类锌离子增强型荧光探针的合成及光谱性能
Synthesis and Spectral Properties of Zinc Ion-enhanced Fluorescence Probe Based on Naphthoyl Acylhydrazone
摘要
Abstract
A simple fluorescent probe HM based on acylhydrazone structure was synthesized for rec-ognition of zinc ion ( Zn2+) . The recognition process of Zn2+ was studied by ESI-MS, fluorescence spectroscopy and UV-Vis spectroscopy. UV-Vis titration test shows that the addition of Zn2+ into HM aqueous solution caused gradually drop of the absorption peak at 386 nm. At the same time, a new absorption peak at 420 nm appeared and the intensity gradually increased until equilibrium. A sharp isosbestic point at 396 nm was obtained. Florescence spectroscopy indicates that the fluorescent probe HM exhibites high selectivity for detection of Zn2+. The fluorescence intensity was increased by 2. 5 times at excited wavelength of 510 nm. The lowest detection limit of HM for Zn2+ is up to 1 . 0 × 10 -5 mol/L and the fluorescence quantum yield is 0 . 02 . The recognition process is presumed to be caused by PET ( photoinduced electron transfer) process combining with CHEF ( Chelation en-hanced fluorescence) mechanism. The Job's plot and ESI-MS results demonstrate a 1:1 stoichiomet-ric host-guest complex for probe molecular HM and Zn2+, with the association constant being calcu-lated as K=4. 05 × 106 L·mol-1 .关键词
锌离子/荧光探针/酰腙/识别Key words
zinc ion/fluorescence probe/acylhydrazone/recognition分类
数理科学引用本文复制引用
吴红梅,郭宇,曹建芳,吴中立..基于萘酰腙类锌离子增强型荧光探针的合成及光谱性能[J].发光学报,2018,39(5):621-626,6.基金项目
国家自然科学基金(21601075,21606118) （21601075,21606118）
辽宁省自然科学基金(2015020249)资助项目 Supported by National Natural Science Foundation of China(21601075,21606118) （2015020249）
Natural Science Foundation of Liaoning Prov-ince(2015020249) （2015020249）
