福建农业学报2018，Vol.33Issue(4)：341-345,5.
羊传染性脓疱病毒SYBR Green Ⅰ实时荧光定量PCR检测方法的建立
Development and Application of a Real-time Fluorescence Quantitative PCR for Detection of Orf Virus
摘要
Abstract
The study established a SYBR Green I real-time fluorescence quantitative PCR assay for detecting orf virus .Using Beacon Designer 7.9 software to design a pair of specific primers based on the VIR gene of the orf virus in the Genbank (accession number . KF666563.1) .The result showed that it would specifically detect orf virus , but wouldn't detect other sheep and goat bacteria and viruses . The detection limit of the method was 100 copies ·μL -1 and the detected Ct values of intra-and inter-variation were all less than 2％ .Using the assay to detect 39 clinical samples , the positive rate was 94.9％ (37/39) .The above results indicated that the assay had a high specificity ,sensitivity and repeatability , which could be used to detect orf virus and conduct epidemiological investigation .关键词
羊传染性脓疱病毒/SYBR Green Ⅰ/实时荧光定量PCRKey words
orf virus/SYBR Green Ⅰ/real-time fluorescence quantitative PCR分类
农业科技引用本文复制引用
林裕胜,江锦秀,张靖鹏,江斌,游伟,胡奇林..羊传染性脓疱病毒SYBR Green Ⅰ实时荧光定量PCR检测方法的建立[J].福建农业学报,2018,33(4):341-345,5.基金项目
国家重点研发计划项目 (2016YFD0500906) （2016YFD0500906）
福建省科技计划项目——省属公益类科研院所基本科研专项 (2018R1023-9) （2018R1023-9）
福建省财政专项——福建省农业科学院青年创新团队 (STIT2017-3-10) （STIT2017-3-10）
福建省农业科学院科技创新项目 (PC2018-6) （PC2018-6）
