福建农业学报2018，Vol.33Issue(4)：351-356,6.
罗非鱼无乳链球菌SIP微胶囊口服疫苗免疫效果研究
Immunological Efficacy of Streptococcus agalactiae SIP Oral Vaccine on Tilapia (Oreochromis niloticus)
摘要
Abstract
The immunological efficacy of Streptococcus agalactiae surface immunogenic protein (SIP) oral vaccine on tilapia (Oreochromis niloticus) was studied .Randomly grouped fish were injected with the inactivated vaccine as the positive control (Group Inactivated Vaccine) ,or orally administered with SIP vaccine (Group SIP Vaccine) for treatment or PBS as the negative control (Group Negative Control) .Spleen samples from the tilapias were collected at 0 ,7 ,14 and 28 d after the immunization .The gene expressions of the cluster determinant 4 (CD4) ,interleukin-8 (IL-8) ,interleukin-10 (IL-10) ,tumor necrosis factor α(TNF-α) and nuclear transcription factors κB (NF-κB) were determined by the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) .Four weeks after the treatments ,the groups were challenged by the artificial infection of S .agalactiae to observe the relative percent survivals(RPS)onthefish.TheresultsshowthattheexpressionsonCD4,IL-8,IL-10,TNF-αandNF-κBgenes of Group SIP Vaccine and Group Inactivated Vaccine increased in varying degrees ,and were significantly higher (P<0.05) than those of Group Negative Control .The death rates of fish in Group SIP Vaccine and Group Inactivated Vaccine were significantly lower (P<0.05) than that of Group Negative Control .The RPS of Group Inactivated Vaccine was higher than that of Group SIP Vaccine ,but not significantly different (P>0.05) .It appeared that the SIP oral vaccine could be used with satisfaction in protecting tilapias from S .agalactiae infection .关键词
罗非鱼/无乳链球菌/表面免疫蛋白/免疫相关基因/免疫保护Key words
tilapia/Streptococcus agalactiae/surface immunogenic protein/immunity-related genes/immunological efficacy分类
农业科技引用本文复制引用
林楠..罗非鱼无乳链球菌SIP微胶囊口服疫苗免疫效果研究[J].福建农业学报,2018,33(4):351-356,6.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项 (2014R1002-3) （2014R1002-3）
福建省海洋与渔业厅重点项目 (闽海渔合同[2010 ] 2-12号) （闽海渔合同[2010 ] 2-12号）
