福建农业学报2018，Vol.33Issue(4)：386-390,5.
台湾速生杨树离体再生体系的建立
Tissue Culture Regeneration of Fast-growing Taiwan Poplar
摘要
Abstract
A regeneration protocol was established using the stems and leaves of fast-growing Taiwan Poplar 3 as explants .The selected disinfection time for the stems was 10 min in a 0.15％ HgCl2 solution to result in an infection rate of 23.3％ and a budding rate of 75.3％ .The optimized medium for generating adventitious buds was MS+BA 0.3mg·L -1 + NAA 0.1mg·L -1 to reach an average differentiation rate of 77.88％ ;that for the leaf differentiation ,MS+BA 0.5 mg·L -1 + NAA 0.2 mg·L -1 + KT 0.2 mg·L -1 + adenine 40 mg·L -1 with a differentiation rate of 87.7％ ;that for the proliferation ,MS+BA 0.5 mg·L -1 + KT 0.3 mg·L -1 + NAA 0.2 mg·L -1 ;and that for rooting ,1/2MS+NAA 0.2 mg·L -1 +CCC 0.5 mg·L -1 with a 100％ rooting rate .关键词
台湾速生杨树3号/组织培养/植株再生Key words
Fast-growing Taiwan Poplar 3/tissue culture/regeneration system分类
农业科技引用本文复制引用
黄枝英,庄卫东,汤红玲,马晓娟,刘靓..台湾速生杨树离体再生体系的建立[J].福建农业学报,2018,33(4):386-390,5.基金项目
泉州市科技计划重点项目 (2011N6) （2011N6）
