福建农业学报2018,Vol.33Issue(4):413-417,5.
一株热噬淀粉芽孢杆菌的分离和鉴定
Isolation and Identification of Bacillus thermoamylovorans
摘要
Abstract
This study aimed to identify microbes that could , at high temperatures , efficiently degrade waste materials from livestock and poultry slaughterhouses . A stepwise gradient heating was applied to screen microorganisms and isolated HL8103 . Through colony morphology , strain morphology , physiological and biochemical characteristics ,16S rDNA sequence ,phylogenetic analysis .At the same time ,the strain was tested for its suitability for livestock and poultry slaughterhouse waste . The results identified HL8103 as Bacillus thermoamylovorans ,a gram-positive with straight or curved rod-shape bacterium that had near spherical spores , proximal birth ,and inflated cysts .Meanwhile ,HL8103 was tested for its adaptability on the slaughterhouse waste and found that it could grow well between 75℃ and 85℃ and withstand a temperature as high as 95℃ .And ,for optimal results ,the medium was to be adjusted to pH 6.8-7.2 and fermented for 18 h .关键词
热噬淀粉芽孢杆菌/分离/鉴定Key words
Bacillus thermoamylovorans/separation/identification分类
生物科学引用本文复制引用
缪伏荣,董志岩,陈鑫珠,刘景..一株热噬淀粉芽孢杆菌的分离和鉴定[J].福建农业学报,2018,33(4):413-417,5.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项 (2015R1101) (2015R1101)