分析化学2018，Vol.46Issue(6)：843-850,8.DOI:10.11895/j.issn.0253-3820.171476
纳米孔道单分子电化学信号在线识别与分析研究
Real-time Event Recognition and Analysis System for Nanopore Study
摘要
Abstract
To achieve fast and accurate analysis of weak current signal of nanopore-based single molecule detection, we designed a real-time adaptive threshold data processing algorithm with data buffering technique and finite impulse response filtering. The system, which is designed based on the data processing algorithm, could realize real-time recognition and analysis of nanopore events during the data recording process. In order to verify the performance of the system, the ideal signals with different noise level (20-100 pA) and recording bandwidth (3 - 100 kHz) was generated. The results showed that the system was stable to analyze the generated signals even at high noise. In addition, the system was also suitable for the data recording conditions of low bandwidth and high sampling rate (250 kHz). The proposed nanopore data processing system was further applied in the Aerolysin nanopore experiment for the detection of poly(dA) 4 molecules. The results showed that the data processing system could be applied in real nanopore recording system with high accuracy and speed.关键词
纳米孔道/实时采集存储/在线分析处理/双缓冲区/自适应阈值法Key words
Nanopore/Data acquisition and storage/Real-time data analysis and processing/Data buffering/Adaptive threshold algorithm引用本文复制引用
王慧锋,黄飞,顾震,胡正利,应佚伦,颜秉勇,龙亿涛..纳米孔道单分子电化学信号在线识别与分析研究[J].分析化学,2018,46(6):843-850,8.基金项目
本文系国家自然科学基金(Nos. 21327807, 21421004)、上海市优秀学科带头人计划(No. 15XD1501200) 和中央高校基本科研业务费专项资金(Nos. 222201718001, 222201717003)资助项目 This work was supported by the National Natural Science Foundation of China (Nos. 21327807, 21421004), the Program of Shanghai Subject Chief Scientist (No. 15XD1501200) and the Fundamental Research Funds for the Central Universities (Nos. 222201718001, 222201717003). （Nos. 21327807, 21421004）
