法医学杂志2018，Vol.34Issue(2)：157-160,4.DOI:10.3969/j.issn.1004-5619.2018.02.011
联苯胺试验对血痕DNA检验的影响
Effect of Benzidine Test on DNA Analysis of Bloodstain
金明 1巴华杰 1朱爱华 1马骏 1石津玮 1刘亚楠 2林子清3
作者信息
- 1. 常州市公安局物证鉴定所,江苏 常州 213003
- 2. 上海市公安局物证鉴定中心 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室,上海 200083
- 3. 中国刑警学院法医学系,辽宁 沈阳 110035
- 折叠
摘要
Abstract
Objective To explore the effect of benzidine test and related reagents on DNA analysis of bloodstain. Methods A total of 970 bloodstain filter paper samples with 1μL venous blood were collected, and 10 of them acted as control samples. After benzidine test and related reagent processing, DNA of 960 samples was extracted by Chelex-100 and silica bead methods and then multiplex amplified by AmpF(e)STRTM IdentifilerTM Plus PCR kits. The results of STR typing were compared between different groups. Results DNA were extracted immediately after benzidine test. Totally STR loci (3.80±1.34) were detected by silica bead method, while no STR loci were obtained by Chelex-100 method. Thirteen sam-ples (21.7％) with whole STR typing results were obtained by drying after benzidine test, and the STR locus number (12.90±1.49) which obtained by silica bead method was much higher than by Chelex-100 method (4.70±1.96) (P<0.05). When DNA was extracted immediately after the addition of glacial acetic acid, the STR locus number was (9.40±2.09) by silica bead method, but no STR typing result was obtained by Chelex-100 method. All 15 STR loci could be obtained by only adding glacial acetic acid after drying and only adding tetramethylbenzidine alcoholization liquid or 3％ hydrogen peroxide liquid. Conclusion Benzidine test has significant influence on DNA analysis of bloodstain. The Chelex-100 method is not suitable for the DNA extraction of bloodstain after benzidine test. Drying after benzidine test and silica bead methods can effectively enhance the STR locus number of bloodstain.关键词
法医遗传学/联苯胺试验/血迹/DNA提取/STR分型Key words
forensic genetics/benzidine test/blood stains/DNA extration/STR typing分类
社会科学引用本文复制引用
金明,巴华杰,朱爱华,马骏,石津玮,刘亚楠,林子清..联苯胺试验对血痕DNA检验的影响[J].法医学杂志,2018,34(2):157-160,4.
