干旱地区农业研究2018，Vol.36Issue(2)：118-123,6.DOI:10.7606/j.issn.1000-7601.2018.02.18
60Co-γ辐射诱变处理对无芒雀麦种子萌发及幼苗期抗旱性的影响
Effect of drought resistance of Bromes irradiated by 60Co-γ-ray in the seed germination stage
摘要
Abstract
In order to explain the effect of water tolerance of 60 Co-γradiation on the seed germination of Bromes , polyethylene glycol (PEG-6000 ) was used to simulate water stress ,The seeds of Bromes are used as materials ,using germination box filter paper germination .the effects of different 60Co-γ radiation on the seeding growth、cell membrane permeability、osmotic adjustment and the activity of protective enzymes were studied .The results showed that :10％ PEG was the best stress concentration in the seed germination period of Bromes .Low dose radiation to improve the root of the seed water absorption capacity ,and promote the water content of seedling ,there by promotion seedling growth ,high-dose radiation is inhibited .But the relative conductivity content was positively correlated with the radiation dose ,the radiation dose gradually increased ,the destruction of the cell membrane is enhanced .The contents of proline ,soluble sugar and soluble protein were negatively correlated with the radiation dose .Low dose radiation could promote the osmotic adjust-ment of substance and maintain normal physiological metabolism .With the increase of radiation dose ,the protective en-zyme activities of Bromes were all increased first and then decreased ,which indicated that low dose radiation could pro-mote the free radical scavenging ability of Bromes ,and the high dose had inhibitory effect .Low dose radiation can pro-mote promotes its ability of scavenging radicals of Bromes ,while the high dose has inhibitory effect .Thus ,the low dose of 60 Co-radiation (50～100 Gy ) to promote the growth of Bromes and protected the cell membrane by the decrease of relative conductivity .At the same time ,there is no increase in osmotic regulation and antioxidant enzyme activity to adapt to the water stress environment Inhibition ,the degree of damage to a minimum .关键词
无芒雀麦/PEG胁迫/60Co-γ辐照/抗旱性Key words
bromes/PEG stress/60 Co-γ- ray/drought resistance分类
农业科技引用本文复制引用
李波,李祥莉..60Co-γ辐射诱变处理对无芒雀麦种子萌发及幼苗期抗旱性的影响[J].干旱地区农业研究,2018,36(2):118-123,6.基金项目
黑龙江省应用技术研究与开发计划重大项目(GA15B105-5) （GA15B105-5）
黑龙江省教育厅基本业务专项(135209267) （135209267）
