医学分子生物学杂志2018，Vol.15Issue(3)：149-155,7.DOI:10.3870/j.issn.1672-8009.2018.03.006
前列腺癌与前列腺增生的差异基因比较
Differences in Gene Expression between Prostate Cancer and Benign Prostatic Hyperplasia
摘要
Abstract
Objective To compare the differentially expressed genes between prostate cancer and benign prostatic hyperplasia ( BPH) in an attempt to provide diagnostic value for prostate canc-er and BPH. Methods Agilent human genome-wide expression profiling microarray (4 × 44 K) was used to screen differentially expressed genes in prostate cancer and BPH tissues, and the differ-entially expressed genes were analyzed by cluster analysis, GO and KEGG pathway enrichment. Results The expression profiling analysis showed that there were 327 differentially expressed genes in prostate cancer tissues ( P<0. 05; Fold change >2 ) compared with those in BPH tissues, of which 138 genes were up-regulated and 189 genes were down-regulated. GO enrichment analysis re-vealed that the down-regulated differential genes were mainly involved in T cell receptor synthesis, integrin-mediated cell adhesion, B cell differentiation, dendritic cell chemotaxis and migration, and formation of immune synapses. KEGG analysis showed that the differential genes were mainly involved in innate immune deficiency, taurine metabolism, histidine metabolism, and B cell re-ceptor signaling pathways. Conclusion Gene expression microarray of prostate cancer and BPH showed that differentially expressed genes are mainly involved in the formation of immune cells and the meditation ofin-vivoimmune responses. Analyses of differentially expressed genes between pros-tate cancer and BPH can provide new predictors and new targets for the diagnosis and treatment of prostate cancer. They also provide valuable clinical data for the study of prostate cancer.关键词
前列腺癌/前列腺增生/基因芯片Key words
prostate cancer/benign prostatic hyperplasia/genome microarray分类
生物科学引用本文复制引用
张朝晖,夏宇,杨安卿,黄滔,楚晨龙,赵晨晖,马斌斌,崔仁杰,周文龙..前列腺癌与前列腺增生的差异基因比较[J].医学分子生物学杂志,2018,15(3):149-155,7.基金项目
上海市黄浦区科委科技项目(No.LKW1105) （No.LKW1105）
This work was supported by a grant from the Shanghai Huangpu District Science and Technology Projects (No. LKW1105) （No. LKW1105）
