医学分子生物学杂志2018，Vol.15Issue(3)：156-160,5.DOI:10.3870/j.issn.1672-8009.2018.03.007
白藜芦醇联合姜黄素对肝癌细胞凋亡的机制研究
Effects of Resveratrol Combined with Curcumin on Apoptosis of Liver Cancer Cells
周思聪 1刘力华2
作者信息
- 1. 浙江中医药大学第三临床医学院 杭州市, 310053
- 2. 海宁市康华医院肿瘤科 浙江省海宁市, 314400
- 折叠
摘要
Abstract
Objective To explore the effects of resveratrol in combination with curcumin on apoptosis of liver cancer cells. Methods Liver cancer cell line SMMC-7721 was used and divided into 4 groups, including group A (control group), group B (resveratrol group), group C (cur-cumin group) and group D (resveratrol+curcumin group) . CCK-8 (cell counting kit-8) method was used to detect the proliferative rates in different groups; colorimetry was employed to detect the activities of caspase-3 , caspase-8 and caspase-9; Western blotting was utilized to detect the expres-sion of Bax, Bcl-2, PARP ( poly-ADP-ribose polymerase) and Cleaved PARP. Results CCK-8 results showed that the proliferative rate was lowest in group D ( P<0. 05 ) . Colorimetric results exhibited that the activity of caspase-3 , caspase-8 and caspase-9 was highest in group D ( P <0. 05) . Western blotting revealed that Bax expression in group D was higher, and Bcl-2 expression was lower in group D than in group A, B and C ( P<0. 05 for all) . PARP expression was lower in group D and cleaved PARP was higher in group D than in group A, B and C (P<0. 05) . Con-clusion Resveratrol and curcumin can induce the apoptosis in SMMC-7721 cells, and the combi-nation of both can enhance the effects, possibly by increasing the caspase activity and Bax expres-sion, and decreasing Bcl-2 expression and the cleavage of PARP.关键词
白藜芦醇/姜黄素/肝癌/凋亡Key words
resveratrol/curcumin/liver cancer/apoptosis分类
医药卫生引用本文复制引用
周思聪,刘力华..白藜芦醇联合姜黄素对肝癌细胞凋亡的机制研究[J].医学分子生物学杂志,2018,15(3):156-160,5.
