解放军医药杂志2018，Vol.30Issue(5)：1-4,4.DOI:10.3969/j.issn.2095-140X.2018.05.001
大鼠骨髓间充质干细胞的分离、鉴定与冻存及对细胞活性的影响
Separation, Identification and Cryopreservation of Bone Marrow Mesenchymal Stem Cells in Rats and Their Effects on Cytoactive
摘要
Abstract
Objective To separate and culture bone marrow mesenchymal stem cells (MSCs), and to observe effect of cryopreservation on cytoactive so as to provide foundation for its clinical application. Methods SD male rats with 3 weeks old were selected and killed by decollation, and thighbone and tibia were used to extract MSCs for culture in vitro. Positive and negative markers on MSCs surfaces were detected by flow cytometry. All selected MSCs were stored under - 70℃ circumstance, and before and in 1 week, 1 month and 6 months after cryopreservation the cells were re-vived, and then MTT assay was used to draw cumulative growth curve, and differentiated capacities of osteoblast and adi-pocyte were detected by induced assay. Results The positive rates of MSCs superficial positive markers CD29 and CD90 were 97. 9％ and 97. 4％ respectively, and reanimation rates of MSCs in 1 week, 1 month and 6 months after cryopreser-vation were no significant differences among groups (P > 0. 05). There were no significant differences in effects of cryopr-eservation time and prolonging cryopreservation time on cell multiplication activity and multi-differentiation ability, num-bersof calcium nodus and oil drop after condition induction between groups (P > 0. 05). Conclusion In this experiment,bone marrow MSCs in rats are extracted successfully, and the frozen storage system may maintain MSCs activity for a long time, which provides a foundation for subsequent study.关键词
骨髓/间充质干细胞/低温保存/细胞活性Key words
Bone marrow/Mesenchymal stem cells/Cryopreservation/Cytoactive分类
医药卫生引用本文复制引用
谢晶晶,李博,姚兵,宋烜,张平超,陆骅..大鼠骨髓间充质干细胞的分离、鉴定与冻存及对细胞活性的影响[J].解放军医药杂志,2018,30(5):1-4,4.基金项目
上海市卫生局青年科研项目(201244151) （201244151）
