解放军医药杂志2018，Vol.30Issue(5)：5-8,14,5.DOI:10.3969/j.issn.2095-140X.2018.05.002
长链非编码RNA-GAS5对人单核-巨噬细胞THP-1凋亡的影响
Effect of LncRNA-GAS5 on Human Mononuclear Macrophage Apoptosis
摘要
Abstract
Objective To investigate effects of LncRNA-GAS5 knockdown or overexpression on human mononu-clear macrophage apoptosis. Methods Knockdown or overexpressed LncRNA-GAS5 of human mononuclear macrophage cell lines THP-1 were established by using siRNA or LncRNA-GAS5 expression plasmids. Conditions of cell apoptosis were detected using Annexin V-PE/ 7-AAD kits and flow cytometry methods. Western blot was used to detect expression of associated apoptotic protein. Results Apoptotic percentage of THP-1 cells in low-expressed LncRNA-GAS5 group was significantly higher than that in normal-expressed LncRNA-GAS5 group. There was no significant difference in apoptotic percentage of THP-1 cells between over-expressed and normal-expressed LncRNA-GAS5 groups; while under ox-LDL (75 μg/ ml, 24 h) stimulation, apoptotic percentage of THP-1 cells in low-expressed LncRNA-GAS5 group was signifi-cantly increased compared with that in normal-expressed LncRNA-GAS5 group. Apoptotic percentage of THP-1 cells in o-ver-expressed LncRNA-GAS5 group was significantly decreased compared with that in normal-expressed LncRNA-GAS5 group. Analysis of apoptotic protein showed that under ox-LDL (75 μg/ ml, 24 h) stimulation, overexpression of Ln-cRNA-GAS5 significantly decreased protein expressions of caspase-9, caspase-7 and caspase-3, while knockdown of Ln-cRNA-GAS5 significantly promoted the above protein expressions. Conclusion LncRNA-GAS5 may obviously inhibit hu-man mononuclear macrophage apoptosis.关键词
长链非编码RNA-GAS5/单核-巨噬细胞/凋亡Key words
LncRNA-GAS5/Mononuclear macrophage/Apoptosis分类
医药卫生引用本文复制引用
陈磊,杨文进,陈伟,郑平,郭义君,曾劲松,童武松..长链非编码RNA-GAS5对人单核-巨噬细胞THP-1凋亡的影响[J].解放军医药杂志,2018,30(5):5-8,14,5.基金项目
浦东新区科技发展基金项目(PKJ2015-Y29) （PKJ2015-Y29）
浦东新区卫生系统优秀青年医学人才培养计划(PWRq2016-29) （PWRq2016-29）
浦东新区卫生、计生科技项目(PWZz2013-13) （PWZz2013-13）
