解放军医药杂志2018，Vol.30Issue(5)：9-14,6.DOI:10.3969/j.issn.2095-140X.2018.05.003
丝裂原活化蛋白激酶磷酸酶1在糖尿病大鼠肾组织细胞外基质重构中的作用
Effect of Mitogen-activated Protein Kinase Phosphatase-1 in Extracellular Matrix Reconstruction of Renal Tis-sues in Rats with Diabetes Mellitus
摘要
Abstract
Objective To investigate expression characteristics of mitogen-activated protein kinase phosphatase-1 (MKP-1) in renal tissues of rats with diabetic mellitus (DM) and its effect on extracellular matrix (ECM) reconstruc-tion. Methods A total of 60 male Wister rats were used to establish diabetic models by injecting Streptozotocin (STZ, 65 mg/ kg) intraperitoneally for one time. At 48 h after injection, rats with blood glucose equal or larger than 16. 7 mmol/ L and 3 + -4 + harnzucker were selected as DM group, and then rats were respectively sacrificed in the 1st , 2nd , 4th , 8th and 12th weeks after successful model establishment for 10 rats in one time, and other 10 rats were selected as control group. Immunohistochemistry test was used to detect expressions of MKP-1, p38 mitogen-activated protein ki-nase (p38 MAPK) and laminin (LN) in renal tissues. Western blot method was used to detect expressions of MKP-1 and phosphorylation p-p38 MAPK, and reverse transcription-polymerase chain reaction (RT-PCR) was used to detect expres-sions of MKP-1 and fibronectin (FN) mRNA. Results Compared with those in control group, expressions of MKP-1 protein and mRNA renal tissues of rats with diabetic mellitus in the 1st-8th weeks after successful model establishment were significantly decreased (P < 0. 01); phosphorylation p-p38 MAPK expressions in the 2nd-8th weeks after successful model establishment were significantly increased ( P < 0. 01), while the phosphorylation p-p38 MAPK expression in the 12th weeks after successful model establishment showed no significant difference (P > 0. 05); LN expressions were gradually increased in the 4th-12th weeks compared with those in control group (P < 0. 01); FN mRNA expressions were significant-ly increased from the 2nd to 12th weeks (P < 0. 01). Conclusion MKP-1 expression can be found decreased in renal tis-sues of rats with diabetic mellitus, and it plays a certain role in pathogenesis of extracellular matrix reconstruction, which may be related to dephosphorylation of p38 MAPK pathway.关键词
糖尿病肾病/丝裂原活化蛋白激酶磷酸酶类/细胞外基质/大鼠,WisterKey words
Diabetic nephropathies/Mitogen-activated protein kinase phosphatases/Extracellular matrix/Rats/Wister分类
医药卫生引用本文复制引用
王丽晖,吴广礼,林静,黄旭东,杨新军,汪晶华,陈云爽,赵维..丝裂原活化蛋白激酶磷酸酶1在糖尿病大鼠肾组织细胞外基质重构中的作用[J].解放军医药杂志,2018,30(5):9-14,6.基金项目
河北省自然科学基金资助项目(H2016206578) （H2016206578）
