湖南农业大学学报（自然科学版）2018，Vol.44Issue(3)：261-270,10.DOI:10.13331/j.cnki.jhau.2018.03.007
忍冬属黄酮合成酶基因FNSII的SNP分析
SNP analysis of flavone synthase Ⅱ gene (FNSII) in Lonicera species
摘要
Abstract
Potential single nucleotide polymorphisms (SNPs) of FNSII gene is helpful to identify Lonicera japonica and Lonicera confuse. Previously, 12 samples had been identified into five Lonicera species (Lonicera japonica Thunb.,Lonicera macranthoides Hand.-Mazz., Lonicera hypoglauca Miq., Lonicera fulvotomentosa, Lonicera confusa) in the base of base resource. Here, we have performed further study on these 12 samples. First, homology-based cloning was employed to clone their FNSII genes, and then SNP analysis. To our knowledge, our study is the first time to obtain the FNSII genesequences from Lonicera hypoglauca Miq., Lonicera fulvotomentosa and Lonicera confusa. The similarity of all the cloned 20 FNSII gene fragments is over 95％, the phylogenetic analysis shows that genetic relationship of these Lonicera L. is consistent with the identification results. 38 SNPs were identified from 20 sequences in 12 samples via sequence alignment.According to the difference of SNPs, all samples can be classed into 5 genotypes according to the total 38 SNPs, which were identified from 20 sequences cloned in 12 samples and 19 of which can be used for the species indenfication.关键词
忍冬属/金银花/山银花/黄酮合成酶基因/SNP位点Key words
Lonicera/Lonicera japonica/Lonicera confuse/flavone synthase Ⅱ gene(FNSII)/SNP sites分类
农业科技引用本文复制引用
何志敏,扈麟,聂平,钟庆元,鲁双庆,李文莉..忍冬属黄酮合成酶基因FNSII的SNP分析[J].湖南农业大学学报（自然科学版）,2018,44(3):261-270,10.基金项目
湖南省自然科学基金项目(2017JJ3183) （2017JJ3183）
湖南省药品检验研究院孵化基金重点项目(2017F001) （2017F001）
