湖南农业大学学报（自然科学版）2018，Vol.44Issue(3)：271-277,7.DOI:10.13331/j.cnki.jhau.2018.03.008
烟草NtGRAS基因的克隆与转录激活及表达特性分析
Clone of NtGRAS gene in tobacco and its transcriptional activation and expression analysis
摘要
Abstract
In this study, a novel GRAS transcription factor gene named NtGRAS was isolated by silico cloning from Ncotiana tabacum (NC89). The cloned complete ORF of NtGRAS was 1311 bp, for a protein of 436 amino acids.Bioinformatics analysis indicated that NtGRAS with molecular weight 47781, isoelectric point 5.16 and located in the nucleus. It shared high homology with AtSCL23 in Arabidopsis thaliana by the phylogenetic analysis. A transactivation assay in yeast cells demonstrated that the NtGRAS protein possessed a transcriptional activation activity. Real-Time PCRanalysis indicated that NtGRAS expressed in roots, stems, leaves and flowers, especially in roots. In addition, NtGRAS expression was up-regulated by low temperature (4 ℃), NaCl (200 μmol/L) and drought treatments.关键词
烟草/NtGRAS/转录激活/基因表达Key words
tobacco/NtGRAS/transcriptional activation/gene expression分类
农业科技引用本文复制引用
杨辉,杨晓娜,王翀,卢向阳,田云..烟草NtGRAS基因的克隆与转录激活及表达特性分析[J].湖南农业大学学报（自然科学版）,2018,44(3):271-277,7.基金项目
高等学校博士学科点专项科研基金项目(20134320110010) （20134320110010）
湖南省自然科学基金重点项目(13JJ2028) （13JJ2028）
湖南省教育厅重点项目(13A041) （13A041）
湖南省研究生科研创新项目(CX2015B240) （CX2015B240）
