湖南农业大学学报（自然科学版）2018，Vol.44Issue(3)：278-283,6.DOI:10.13331/j.cnki.jhau.2018.03.009
水稻种子特异表达基因OsEnS38的克隆与表达
Cloning and expression analysis of a seed-specific gene OsEnS38 in rice
摘要
Abstract
In this study, we identified and cloned the OsEnS38 cDNA sequence in Zhonghua 11 using bioinformatic and RT-PCR analysis. The encoded OsEnS38 contains two conservative cupin_1 domain typical in Bicupin superfamily.Promoter sequence analysis revealed the existence of several confirmed endosperm-specific cis-regulatory elements in the OsEnS38 promoter. It was confirmed by qRT-PCR and in situ hybridization analysis that the expression of OsEnS38was specifically expression in developing seeds 7-14 days after pollination. Further analysis revealed that OsEnS38 together with its co-expressing genes were liable to participate in post-embryonic development and reproduction. The results suggested that it might play a role in seed development and accumulation of storage.关键词
水稻/种子特异表达基因/基因克隆/原位杂交/共表达分析Key words
rice/seed-specific gene/gene cloning/in situ hybridization/co-expression分类
农业科技引用本文复制引用
张焕,仇忠凯,阎新,欧阳解秀,李绍波,王鑫..水稻种子特异表达基因OsEnS38的克隆与表达[J].湖南农业大学学报（自然科学版）,2018,44(3):278-283,6.基金项目
国家自然科学基金项目(31760080,31560298) （31760080,31560298）
