湖南农业大学学报（自然科学版）2018，Vol.44Issue(3)：284-289,6.DOI:10.13331/j.cnki.jhau.2018.03.0010
烟草和拟南芥表皮毛中特异表达iaaM后的遗传效应
The genetic effect analysis of iaaM specific expressed in trichome in tobacco and Arabidopsis
摘要
Abstract
The iaaM, a tryptophan monooxygenase gene deriving from bacteria, can catalyze the synthesize of indole acetic acid (IAA) with tryptophan via indoleacetamide pathway in plants. A trichome specific expression of iaaM vector by recombination of the trichome specific GL2 gene promoter of Arabidopsis and iaaM gene coding region was constructed. The recombinant was transformed into tobacco WS38 by Agrobacterium tumefaciens mediated leaf disc transformation and 6 transformed tobacco plantlets were screened and identified. A comparative experiment was transformation of Arabipdopsis by the Agrobacterium tumefaciens infiltration method with the same recombinant and 9transformed seedlings were screened out. A systematic observation of the epidermal trichome of stably transformed transgenic plants showed that the trichome of transgenic tobacco grew significantly longer than those of control plantswith the length 2.2 times than that of the control. However, there was no significant increase in the length of trichome intransgenic arabidopsis. The IAA content in young leaf of the iaaM transgenic tobacco was significantly increased indicating that iaaM was expressed in tobacco trichome cells so that it achieved more auxin synthesis and accumulation in trichome cells. It was interesting that there was a significant increase in trichome density both in transgenic Arabidopsis and tobacco. It might existed a more complex correlation between the specificity of the GL2 promoter expression in trichome determination and the auxin homeostasis. The mechanism of the trichome elongation in transgenic tobacco was also deduced.关键词
烟草/拟南芥/色氨酸单加氧酶基因/表皮毛/生长素/遗传效应Key words
tobacco/Arabidopsis/tryptophan monooxygenase gene(iaaM)/trichome/auxin/geneticeffect分类
生物科学引用本文复制引用
谢德雄,潘嘉立,赵燕,黄丽华,黄妤,陈金军,张学文..烟草和拟南芥表皮毛中特异表达iaaM后的遗传效应[J].湖南农业大学学报（自然科学版）,2018,44(3):284-289,6.基金项目
湖南省教育厅项目(15K060) （15K060）
湖南农业大学东方科技学院大学生创新项目(DFCXY201610) （DFCXY201610）
