华西口腔医学杂志2018，Vol.36Issue(3)：247-251,5.DOI:10.7518/hxkq.2018.03.003
浓缩生长因子促人脐静脉血管内皮细胞成血管化作用研究
Anin vitro study of the angiogenic effects of concentrate growth factor on human umbilical vein endothelial cells
摘要
Abstract
Objective This study aimed to explore the effects of concentrate growth factor extracts (CGFe) on human umbi-lical vein endothelial cells (HUVECs) in vitro. Methods Concentrate growth factor (CGF) were prepared from the peripheral blood of healthy donors, followed by CGFe. Four groups were designed based on cell culture medium, as follows: 2％CGFe, 5％CGFe, 10％CGFe, and control. The proliferation activity of HUVECs was detected by cell cycle and CCK-8 assays. The migration of HUVECs was detected by scratch assay. The mRNA expression levels of vascular endothelial growth factor (VEGF), chemokine receptor 4 (CXCR4), and platelet derived growth factor (PDGF) were examined by quantitative real time polymerase chain reaction (qRT-PCR). Results Results of CCK-8 and cell cycle assays showed that CGFe promoted the proli-feration capability of HUVECs in a dose-dependent manner, and the data had statistical significance among four groups (P<0.05). The cell migration assay indicated that CGF accelerated wound closure in a dose-dependent manner after 12 h of culture (P<0.05). The results of qRT-PCR showed that CGF upregulated the expression levels of VEGF, CXCR4, and PDGF in HUVECs. Conclusion CGFe can promote the proliferation, migration, and angiogenic differentiation of HUVECs. Thus, CGF might be an appropriate cure for dental pulp revascularization.关键词
浓缩生长因子/人脐静脉血管内皮细胞/增殖/分化Key words
concentrate growth factor/human umbilical vein endothelial cells/proliferation/differentiation分类
生物科学引用本文复制引用
宦俊,窦磊,严崎方,杨德琴..浓缩生长因子促人脐静脉血管内皮细胞成血管化作用研究[J].华西口腔医学杂志,2018,36(3):247-251,5.基金项目
国家自然科学基金面上项目(31571508,31371473) （31571508,31371473）
2016年重庆高校创新团队建设计划 （）
重庆市高校市级口腔生物医学工程重点实验室资助项目[渝教科2014(55)号]Supported by: The National Natural Science Foundation of China (31571508, 31371473) （55）
Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016 （）
Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education[2014(55)]. （55）
