华西口腔医学杂志2018，Vol.36Issue(3)：257-261,5.DOI:10.7518/hxkq.2018.03.005
分泌型卷曲相关蛋白1及β-连环蛋白在慢性牙周炎患者牙龈组织中的表达
Expression and significance of secreted frizzled-related protein 1 and β-catenin in gingival tissue of patients with chronic periodontitis
摘要
Abstract
Objective This study aimed to investigate the expression and correlation of secreted frizzled-related protein 1 (SFRP1) and β-catenin in gingival tissues of patients with chronic periodontitis (CP). The role of the classical Wnt/β-catenin signaling pathway in the development of periodontitis was also explored. Methods Twenty-eight patients with CP (CP group) were enrolled in this study. Among them, 16 cases were moderate CP, and 12 demonstrated severe CP. Twelve healthy cases comprised the controls (normal group). Gingival tissue was collected, and the probing depth, bleeding index, and clinical attachment loss were recorded. The expression levels of SFRP1 and β-catenin were detected by immunohistochemistry, and staining intensity was evaluated by double scoring method. SPSS 19.0 was used for statistical analysis. Results The staining strength scores of SFRP1 and β-catenin were 2.16±0.65 and 1.12±0.51 in the normal group, 3.57±0.45 and 2.36±0.49 in the CP group, 3.61±0.40 and 2.30±0.44 in the moderate CP group, and 3.52±0.52 and 2.45±0.55 in the severe CP group, respectively. The expression of SFRP1 and β-catenin in the CP group was higher than that in the normal group (P<0.01). A significant difference was noted between the normal group and the moderate and severe CP groups (P<0.01) but none between the moderate and severe CP groups (P>0.05). A positive correlation was found between the expression of SFRP1 and β-catenin (r=0.657, P<0.01). The expression levels of β-catenin and SFRP1 were related to periodontal indexes. The correlation between the expression of SFRP1 and probing depth was most significant (r=0.723, P<0.01), as well as that between β-catenin and bleeding index (r=0.697, P<0.01). Conclusion Patients with CP exhibit elevated expression of SFRP1 and β-catenin in gingival tissues, and this event is related to the degree of periodontal destruction. Abnormal expression of SFRP1 and β-catenin may promote the development of periodontitis.关键词
慢性牙周炎/分泌型卷曲相关蛋白1/β-连环蛋白Key words
chronic periodontitis/secreted frizzled-related protein 1/β-catenin分类
医药卫生引用本文复制引用
池毓坦,许春姣,孙晓娟,李冬梅,王宏峰,王苗苗,贺秀芳..分泌型卷曲相关蛋白1及β-连环蛋白在慢性牙周炎患者牙龈组织中的表达[J].华西口腔医学杂志,2018,36(3):257-261,5.基金项目
教育部留学回国人员科研启动基金[教外司留(2012)940号]Supported by: The Project-sponsored by Scientific Research Foundation for Returned Overseas Chinese Scholars, State Edu-cation Ministry of China [(2012)940]. （2012）
