渔业科学进展2018，Vol.39Issue(3)：36-43,8.DOI:10.19663/j.issn2095-9869.20170328002
牙鲆抗迟缓爱德华氏菌病家系的继代选育 与早期速生家系筛选
Screening of Disease-Resistant and Fast Growing Families and Subculture Breeding for Resistance Against Edwardsiella tarda in Japanese Flounder (Paralichthys olivaceus)
摘要
Abstract
Edwardsiella tarda is one of the pathogenic bacteria of Paralichthys olivaceus that seriously affects the sustainable development of flounder aquaculture. In the present study, we used the family of high-quality flounder developed in 2012, 2013, and 2014 as the parents, and developed 28 flounder families in 2016, including 4 F3 generation families, 23 F4 generation families, and control family. After 60 days of growth trait assay and artificial bacterial infection experiments, seven families with strong resistance to disease (survival rate > 66％) were screened. F1639 was screened as the F4 generation family with enhanced disease resistance and fast growth rate. Furthermore, its survival rate was 77.23％, which was 32.75％ higher than that of the average of 28 families. The daily growth rate was 0.174 cm/d, which was 0.023 cm/d higher than that of the other 28 families. F1639 was derived from the 2007 (F0750, resistant to Vibrio anguillarum family), 2009 (F0927, resistant to V. anguillarum family), and 2014 (F1421, resistant to E. tarda family) families. The results showed that the disease resistance of the breeding families improved. Furthermore, the breeding ability of the offspring can be improved by the breeding of the family (the selection of compatriots). The present study has laid a foundation for breeding new variety of Japanese flounder with higher disease resistance and growth rate. Furthermore, it has important potential application in the genetic breeding for disease-resistant and fast growth stock in other fish species.关键词
牙鲆/迟缓爱德华氏菌/F4代家系/抗病Key words
Paralichthys olivaceus/Edwardsiella tarda/F4 generation family/Disease-resistance分类
农业科技引用本文复制引用
李泽宇,李文升,王晓梅,陈松林,郭华,王磊,王倩,李仰真,杨英明,刘寿堂,孙德强..牙鲆抗迟缓爱德华氏菌病家系的继代选育 与早期速生家系筛选[J].渔业科学进展,2018,39(3):36-43,8.基金项目
国家自然科学基金(31461163005)、中国水产科学研究院基本科研业务费(2016HY-ZD02)和山东省泰山学者攀登计划项目共同资助[This work was supported by the National Natural Science Foundation of China (31461163005), Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences (2016HY-ZD02), and Taishan Scholar Climbing Project, Shandong]. （31461163005）
